Isolation, characterization, and antifungal susceptibility of melanin-deficient mutants of Scedosporium prolificans
Scedosporium prolificans mutants lacking the ability to synthesize melanin were selected after ultraviolet light (UV) irradiation. UV exposure of S. prolificans conidia resulted in a high frequency of melanin-deficient (mel-) mutants. Stable and non-stable morphological variants were found in the population reversion of the mutant phenotype was always to the wild-type phenotype. Based on their morphological differences, these variants were classified into five different groups that were phenotypically characterized. The mel- mutants plus the wild-type strain were examined for in vitro susceptibility to antifungal agents with different and/or the same mechanism of action. There was no apparent difference in minimum inhibitory concentrations when comparing the wild-type and the mel- mutants. Therefore, melanin does not appear to confer protection against the more important antifungal agents in S. prolificans.
| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Springer
2003
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| Online Access: | http://hdl.handle.net/20.500.12792/3930 http://hdl.handle.net/10261/294487 |
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