Construction of gene interaction and regulatory networks in bovine skeletal muscle from expression data
We propose a data-driven reverse engineering approach to isolate the components of a gene interaction and regulatory network. We apply this method to the construction of a network for bovine skeletal muscle. Key nodes in the network include muscle-specific genes and transcription factors, muscle-specific genes are identified from data mining the USA National Cancer Institute, Cancer Genome Anatomy Project database, while transcription factors are predicted by accurate function annotation. A total of 5 microarray studies spanning 78 hybridisations and 23 different experimental conditions provided raw expression data. A recently-reported analytical method based on multivariate mixed-model equations is used to compute gene co-expression measures across 624 genes. The resulting network included 102 genes (of which 40 were muscle-specific genes and 7 were transcription factors) that clustered in 7 distinct modules with clear biological interpretation. © CSIRO 2005.
| Main Authors: | , , , , , , , |
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| Format: | conference object biblioteca |
| Language: | English |
| Published: |
CSIRO Publishing
2005
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| Online Access: | http://hdl.handle.net/20.500.12792/3695 http://hdl.handle.net/10261/294419 |
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