Different Pr1A proteins increase the efficiency of periplasmic production of human interleukin-6 in Escherichia coli

The export efficiency of a fusion of the Escherichia coli preOmpA signal peptide to human interleukin-6 can be significantly raised by coexpressing three different prlA alleles of secY along with wild type secE. The effect seems prlA-specific, as prlG1 (a prl allele of secE) does not affect the export of preOmpA-hIL-6. Coexpression of secD and secF also stimulates the export of the fusion protein. The export efficiency of a fusion of the Escherichia coli preOmpA signal peptide to human interleukin-6 can be significantly raised by coexpressing three different prlA alleles of secY along with wild type secE. The effect seems prlA-specific, as prlG1 (a prl allele of secE) does not affect the export of preOmpA-hIL-6. Coexpression of secD and secF also stimulates the export of the fusion protein.

Bibliographic Details

Main Authors:	Pérez-Pérez, J., Barbero, J. L., Márquez, G., Gutiérrez, J.
Format:	journal article biblioteca
Language:	English
Published:	Elsevier 1996
Subjects:	Protein secretion, Interleukin-6, set Y,
Online Access:	http://hdl.handle.net/20.500.12792/3472 http://hdl.handle.net/10261/294350
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