In vivo detection of DNA alterations produced by Benzo(a)pyrene using the RAPD technique
The RAPD technique (Random Amplified Polymorphic DNA) is able to detect nonspecific alterations in the DNA of cells with identical gene pool, such as in the case of the established cell lines, by band pattern comparisons of exposed and nonexposed cells. In the past few years, we have developed a sensitive and highly reproducible methodology using RTG-2 cells, an established cell line derived from rainbow trout (Oncorhynchus mykiss). However, it is necessary to test the predictive ability of this assay for in vivo studies. The RTG-2 cell line, as we have previously shown, presents a good genetic similarity with the species from which it derives. Here we show the results of an in vivo study on rainbow trout individuals sublethally exposed to 69 μg/g bw of benzo(a) pyrene by i.p. injection. The genotoxic effects have been assessed by band pattern comparison of the DNA that was obtained from peripheral blood cells taken up at different periods of time (1 - 3 months). Due to the fact that that the presence of polymorphisms interferes with the analysis between the treated and untreated groups, the comparisons were carried out on the same individual before and after the treatment. Qualitative and quantitative analyses showed the presence of new bands and alterations in band intensity, confirming the results obtained in vitro with the same genotoxic agent.
| Main Authors: | , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Asociación Española de Toxicología
2004
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| Online Access: | http://hdl.handle.net/20.500.12792/3118 http://hdl.handle.net/10261/294242 |
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