Antibody response to a fragment of the protein G of VHS rhabdovirus in immunised trout

A fragment (called frg#11, amino acids, aa 56-110) of the protein G (pG) of viral haemorrhagic septicaemia virus (VHSV) was designed after previous results showed it to be recognised by ∼40% of the trout immunised to VHSV [Dis. Aquat. Organ. 34 (1999) 167]. frg#11 was then cloned, expressed, purified and used to study the production of antibodies to its epitopes in trout immunised to VHSV. Anti-frg#11 trout antibodies could be detected in serum from individual trout surviving VHSV exposure, immunised by injection with purified VHSV or DNA-immunised with its pG gene whereas it was not detected in non-infected and non-immunised trout. The trout serum antibodies which reacted more strongly by ELISA using solid-phase frg#11 (continuous or linear epitopes on the sequence of the pG) had the lowest VHSV-neutralising activity (epitopes which are pG conformation-dependent). Because antibodies recognising continuous as well as conformation-dependent epitopes of the pG seem to be involved in protective trout immunological responses to VHSV, the estimation of anti-frg#11 antibodies could help to the dissection of the complex trout antibody response to VHSV infections. In addition, these preliminary results suggest that the determination of anti-frg#11 antibodies might also be used to complement in vitro viral neutralising assays which seem to be restricted to pG conformation-dependent epitopes. © 2002 Elsevier Science B.V. All rights reserved.

Bibliographic Details

Main Authors:	Rocha, A., Fernandez-Alonso, M., Mas, V., Perez, L., Estepa, A., Coll, J. M.
Format:	journal article biblioteca
Language:	English
Published:	Elsevier 2002
Subjects:	Salmonid rhabdovirus, VHSV, Linear epitopes, Trout antibodies, Protein G, Recombinant fragment,
Online Access:	http://hdl.handle.net/20.500.12792/3756 http://hdl.handle.net/10261/294136
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