Sperm selection by Capripure® density-gradient centrifugation versus the dextran swim-up procedure in wild mountain ruminants
This study compares the effectiveness of two methods of sperm selection - Capripure® density-gradient centrifugation (DGC) and dextran swim-up (DSU) - in semen samples from Iberian ibex (Capra pyrenaica) and European mouflon (Ovis musimon). During the increasing photoperiod, Capripure® DGC improved the percentage of sperm with progressive motility (P<0.05) in ibexes, and selected 60.6% of the initial total number of spermatozoa contained in the ejaculate samples. In mouflon, Capripure® DGC selection was unaffected by photoperiod, had no influence on any sperm variable, and selected 47.8% of the initial total number of mouflon spermatozoa in ejaculate samples. Photoperiod had no influence on the effectiveness of DSU in either ibexes or mouflons. In the ibexes, DSU reduced (P<0.05) the percentage of sperm cells with morphological abnormalities, but only selected 11.3% of the initial total number of spermatozoa in ejaculate samples. In the mouflons, DSU had no significant influence on any sperm variable, and selected 27.8% of the initial total number. Capripure® DGC improved ibex and mouflon sperm motility (P<0.05) following 30min and 2h of post-centrifugation, stress-inducing incubation, respectively. In both ibexes and mouflon, sperm cells showing non-progressive motility were found after only 20h of post-centrifugation incubation following Capripure® DGC selection. In conclusion, Capripure® DGC would seem a useful method for selecting the best spermatozoa from both ibex and mouflon ejaculates. © 2014 Elsevier B.V.
| Main Authors: | , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2014
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| Subjects: | Sperm purification, Density gradient centrifugation, Swim-up techniques, Ibex Mouflon, |
| Online Access: | http://hdl.handle.net/20.500.12792/4175 http://hdl.handle.net/10261/294043 |
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