Detection of African horsesickness virus in infected spleens by a sandwich ELISA using two monoclonal antibodies specific for VP7

A sandwich enzyme-linked immunosorbent assay (ELISA) for rapid detection of African horsesickness virus (AHSV) in infected spleens or cell culture supernatant was developed. This method uses two monoclonal antibodies (MAbs) which recognize two non-overlapping epitopes of the major core protein (VP7) to coat the solid phase, and one labeled with biotin as second antibody. This ELISA was evaluated for its ability to detect AHSV in infected spleens resulting in a sensitivity of 97.4% and a specificity of 100% compared with virus isolation in cell culture, and can be used for the detection of the nine different AHSV serotypes. © 1992.

Bibliographic Details

Main Authors:	Laviada, M. D., Babín, M., Dominguez, J., Sánchez-Vizcaíno, J. M.
Format:	journal article biblioteca
Language:	English
Published:	Elsevier 1992
Subjects:	African horsesickness virus, Monoclonal antibody, ELISA, Diagnosis,
Online Access:	http://hdl.handle.net/20.500.12792/5224 http://hdl.handle.net/10261/293803
Tags:	Add Tag No Tags, Be the first to tag this record!