Cereal seed viability after 10 years of storage in active and base germplasm collections
Periodic testing of viability is crucial to operation of seedbanks because it permits the control of genetic erosion during storage. In this work, data obtained at the Spanish Genetic Resource Center (CRF-INIA) from the routine task of monitoring seed viability were analyzed. The accessions studied came from the active and base collections of oat, barley and wheat. All seed entered the cold chambers with a moisture content below 7% and the storage temperatures were -4°C for active collections and -18°C for base collections. Differences between initial and final germination after 10 years of storage were studied in 1770 seed samples 342, 341 and 55 for active collections and 273, 723 and 36 for base collections of oat, barley and wheat, respectively. The percentage of barley and wheat accessions without significant changes in germination ranged from 84% to 89%. Oat seed showed larger deterioration and about 35% of the samples presented significant differences between initial and final germination. Besides, some underestimation of oat initial viability due to seed dormancy was detected. Under the conditions of the CRF seedbank and according to the FAO/IPGRI regeneration standards, 4.6% and 0.4% of the studied active and base samples, respectively, would need a regeneration after 10 years of storage. Possible reasons involved in the loss of viability such as initial seed quality, accession variability and storage conditions are discussed taking into account the available passport and germination information. In our conditions, cultivar variation and storage temperature appeared to be the most influential factors relating to seed storability.
| Main Authors: | , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
1999
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| Subjects: | Cereals, Genetic conservation, Seedbank, Seed viability testing, |
| Online Access: | http://hdl.handle.net/20.500.12792/3915 http://hdl.handle.net/10261/293776 |
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