Determination of β-N-oxalyl-l-α,β-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis

BACKGROUND Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP). Currently, there is no well-defined level below which β-ODAP is considered non-toxic. In this work, the β-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition. RESULTS The level of β-ODAP found in these two species ranged from 0.79 to 5.05 mg g-1. The homoarginine content of the samples ranged from 7.49 to 12.44 mg g-1. CONCLUSION This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of β-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.

Bibliographic Details

Main Authors:	Sacristán, M., Varela, A., Pedrosa, M. M., Burbano, C., Cuadrado Hoyos, María Carmen, Legaz, M. E., Muzquiz, M.
Format:	journal article biblioteca
Language:	English
Published:	Wiley 2015
Subjects:	𝛽B-ODAP, Capillary zone electrophoresis, Homoarginine, Neurolathyrism, Lathyrus sativus, Lathyrus cicera,
Online Access:	http://hdl.handle.net/20.500.12792/3969 http://hdl.handle.net/10261/293762
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