Cryopreservation of epididymal sperm from ibexes (Capra pyrenaica) using short equilibration time with glycerol
Two experiments were conducted to study the effect of shortening the equilibration time with the cryoprotectant glycerol before freezing epididymal sperm recovered postmortem from Iberian ibex. In the first experiment, the standard equilibration time of 3hours was compared with 2hours, and subjective sperm motility and quality of movement were greater (P<0.05) in the latter group. In the second experiment, reducing the equilibration time from 2hours to 15minutes did not affect sperm motility (evaluated subjectively and objectively), viability, acrosomal integrity, or membrane functional integrity. In conclusion, shortening the equilibration time can be used as a technique to simplify the cryopreservation process and this provides practical advantages under field conditions. © 2014 Elsevier Inc.
| Main Authors: | , , , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2014
|
| Subjects: | Epididymal spermatozoa, Postmortem recovery, Cryopreservation, |
| Online Access: | http://hdl.handle.net/20.500.12792/3569 http://hdl.handle.net/10261/293667 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|