LAF1, a MYB transcription activator for phytochrome A signaling
The photoreceptor phytochrome (phy) A has a well-defined role in regulating gene expression in response to specific light signals. Here, we describe a new Arabidopsis mutant, laf1 (long after far-red light 1) that has an elongated hypocotyl specifically under far-red light. Gene expression studies showed that laf1 has reduced responsiveness to continuous far-red light but retains wild-type responses to other light wavelengths. As far-red light is only perceived by phyA, our results suggest that LAF1 is specifically involved in phyA signal transduction. Further analyses revealed that laf1 is affected in a subset of phyA-dependent responses and the phenotype is more severe at low far-red fluence rates. LAF1 encodes a nuclear protein with strong homology with the R2R3-MYB family of DNA-binding proteins. Experiments using yeast cells identified a transactivation domain in the C-terminal portion of the protein. LAF1 is constitutively targeted to the nucleus by signals in its N-terminal portion, and the full-length protein accumulates in distinct nuclear speckles. This accumulation in speckles is abolished by a point mutation in a lysine residue (K258R), which might serve as a modification site by a small ubiquitin-like protein (SUMO).
| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Cold Spring Harbor Laboratory Press
2001
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| Subjects: | Signal transduction, Phytochrome A, Arabidopsis, MYB, Transcription factor, Nuclear speckles, |
| Online Access: | http://hdl.handle.net/20.500.12792/2607 http://hdl.handle.net/10261/293542 |
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