Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation Effect of water activity on accumulation of compatible solutes
Epicoccum nigrum conidia were produced by solid fermentation on wheat grains (cv. Rendeveaux and Brigadier) at different water activities (a(w)). Conidial production was highest at high a(w) (0.996) than at reduced a(w) (0.98). However, conidial production at reduced a(w) was improved when the a(w) of the substrate was adjusted with a mixture of glycerol and water. Maximum levels of conidiation were 7-11 x 106 conidia g-1 grain. The a(w) of the solid substrate affected the pattern of accumulation of compatible solutes in the conidia. Mannitol was the main polyol in all conidial types. However, the amounts of mannitol were higher in conidia produced at high a(w). At reduced a(w) the conidia of E. nigrum accumulated more glycerol, which is more efficient in the osmorregulation proccess than mannitol. Arabitol accumulated in low amounts, specifically in conidia produced at the lower a(w), on cv. Rendeveaux but not on cv. Brigadier. Trehalose was detected in higher amounts in cv. Rendeveaux than in cv. Brigadier, and the amounts were higher in conidia produced at high a(w). A significant amount of endogenous solutes was detected in the washing liquid used for the separation of the conidia.
| Main Authors: | , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Springer
1999
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| Subjects: | Biological control, Compatible solutes, Epicoccum nigrum, Polyols, Solid substrate fermentation, Water activity, |
| Online Access: | http://hdl.handle.net/20.500.12792/3346 http://hdl.handle.net/10261/292969 |
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