Porcine reproductive and respiratory syndrome (PRRS) virus down-modulates TNF-α production in infected macrophages

The effect of porcine reproductive and respiratory syndrome (PRRS) virus infection on the synthesis and secretion of TNF-α and other pro-inflammatory cytokines by porcine alveolar macrophages (PAM) was investigated as well as the effect that TNF-α has on the replication of this virus. A clear reduction of phorbol myristate acetate (PMA)-induced expression of TNF-α mRNA was observed in cells incubated with PRRS virus. Moreover, the presence of PRRS virus also induced a decrease in IL-1 α and MIP-1 β mRNAs expression with respect to PMA-stimulated uninfected cells. According to these results, exposure to the PRRS virus led to a reduction of the TNF-α protein in supernatants of PMA-stimulated PAM. On the other hand, addition of recombinant porcine TNF-α to cultures clearly reduced virus replication; however the addition of TNF-α to cultures containing IFN-α did not result in a further reduction of the produced by IFN-α alone. This indicates the lack of synergy in the effect of these cytokines on viral replication. (C) 2000 Published by Elsevier Science B.V.

Bibliographic Details

Main Authors:	López-Fuertes, L., Campos, E., Doménech, N., Ezquerra Martínez, Ángel, Castro, J. M., Domínguez, J., Alonso, F.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2000
Subjects:	Porcine reproductive and respiratory syndrome, TNF-a, Cytokines,
Online Access:	http://hdl.handle.net/20.500.12792/2468 http://hdl.handle.net/10261/292824
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