Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification

This work examines the effectiveness of a TCG (Tris, citric acid, glucose, 6% egg yolk and 5% glycerol) and a TEST (TES, Tris, glucose, 6% egg yolk and 5% glycerol) sperm extender in the freezing of mouflon spermatozoa at slow cooling rates, using different pre-freezing equilibration times (2–3 hr). It also examines the tolerance of mouflon spermatozoa to different concentrations of cryoprotectants (5, 10, 20% glycerol; 5%, 10%, 20% dimethyl sulfoxide; 6% polyvinylpyrrolidone) and/or sucrose (100, 300, 500 mm). The highest quality (p <.01) thawed spermatozoa were obtained when using the TEST extender and an equilibration time of 3 hr. Sperm motility and membrane integrity were strongly reduced when using rapid freezing rates (60–85°C min−1), independent of the concentration of cryoprotectants. The lowest sucrose concentration (100 mm) provided the highest (p <.05) percentage of motile spermatozoa and live spermatozoa with an intact acrosome. Vitrified–warmed sperm variables were at their best when the spermatozoa was diluted in TCG–6% egg yolk + 100 mm sucrose and warmed at 60°C. Slow warming at 37°C strongly reduced (p <.05) sperm motility and viability. However, sperm vitrification returned lower fertility, sperm motility and sperm viability values than conventional sperm freezing. © 2016 Blackwell Verlag GmbH

Bibliographic Details

Main Authors:	Pradiee, J., Esteso Diez, Milagros Cristina, Castaño García, Cristina, Toledano Díaz, Adolfo, López Sebastián, Antonio, Guerra, R., Santiago Moreno, Julián
Format:	artículo biblioteca
Language:	English
Published:	Wiley 2017
Subjects:	Conventional freezing, Cryopreservation, Spermatozoa, Ultra-rapid freezing, Vitrification,
Online Access:	http://hdl.handle.net/20.500.12792/3568 http://hdl.handle.net/10261/292454
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