Two replication-defective adenoviral vaccine vectors for the induction of immune responses to PPRV

Peste des petits ruminants is a highly contagious disease of small ruminants caused by a Morbillivirus, peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenovirus serotype 5 (Ad5) containing the highly immunogenic fusion protein (F) and hemaglutinine protein (H) genes from PPRV were constructed. HEK293A cells infected with either virus (Ad5-PPRV-F or -H) express F and H proteins respectively. These viruses were used to vaccinate mice by intramuscular inoculation. Both viruses elicited PPRV-specific B- and T-cell responses. Thus, after two immunizations, sera from immunized mice elicited neutralizing antibody response, indicating that this approach has the potential to confer protective immunity. In addition, we detected a significant antigen specific CD4+ and CD8+ T-cell response in mice vaccinated with either virus. These results indicate that these adenovirus constructs offer a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines. © 2013 Elsevier Ltd.

Bibliographic Details

Main Authors:	Rojas Carrasco, José Manuel, Moreno, H., García, A., Ramírez, J. C., Sevilla Hidalgo, Noemi, Martín, V.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2014
Subjects:	PPRV, Vaccines, Adenoviral vectors, Immune response,
Online Access:	http://hdl.handle.net/20.500.12792/3869 http://hdl.handle.net/10261/292442
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