The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Bibliographic Details

Main Authors:	Valckx, S. D. M., De Bie, J., Michiels, E. D., Goovaerts, I. G., Punjabi, U., Ramos Ibeas, Priscila, Gutiérrez Adán, Alfonso, Bols, P. E., Leroy, J. L.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2015
Subjects:	Embryo quality, Follicular fluid, In-vitro maturation, IVF, Oocyte developmental competence,
Online Access:	http://hdl.handle.net/20.500.12792/4608 http://hdl.handle.net/10261/292343
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