Characterization of West Nile virus isolates from Spain New insights into the distinct West Nile virus eco-epidemiology in the Western Mediterranean

West Nile virus (WNV) is a mosquito-borne flavivirus which causes important morbidity and mortality in birds, horses and humans. In the Western Mediterranean region, WNV causes sporadic, self-limited outbreaks, with few or no human cases. Here we report the characterization of two recent Western Mediterranean WNV isolates, obtained in Spain in 2007 from two golden eagles. Complete genome sequence comparisons revealed high identity between these isolates and close relationship with other Western Mediterranean WNV strains isolated since 1996. Phylogenetic analysis within this group indicated that two distinct phylogenetic groups have emerged from earlier strains. Pathogenicity analysis in mice showed that the Spanish isolate is less pathogenic than other strains either from the Western Mediterranean (Morocco 2003) or from North America (NY'99). Changes in amino acid position NS3-249 (claimed as a virulence marker) did not influence the pathogenicity observed. © 2009 Elsevier Inc. All rights reserved.

Bibliographic Details

Main Authors:	Sotelo, E., Fernández Pinero, Jovita, Llorente De Gracia, Francisco, Agüero, M., Hoefle, U., Blanco, J. M., Jiménez-Clavero, M. A.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2009
Subjects:	West Nile virus, Western Mediterranean, Phylogeny, Molecular epidemiology, Complete genome, Virulence, Pathogenicity, Mouse model,
Online Access:	http://hdl.handle.net/20.500.12792/4388 http://hdl.handle.net/10261/292044
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