Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality

BACKGROUND During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. OBJECTIVE To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality. METHODS A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C. RESULTS When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. CONCLUSION The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.

Bibliographic Details

Main Authors:	Tomas, C., Gómez-Fernandez, J., Gómez-Izquierdo, E., Gómez Fidalgo, Ernesto, Sánchez Sánchez, Raúl, González De Bulnes, Antonio, de Mercado, Eduardo
Other Authors:	de Mercado, Eduardo [0000-0003-0321-2431]
Format:	artículo biblioteca
Language:	English
Published:	Cryoletters 2015
Subjects:	Boar sperm, Cryopreservation, Freezing extender, PHsperm quality,
Online Access:	http://hdl.handle.net/10261/291620
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