Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
BACKGROUND During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. OBJECTIVE To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality. METHODS A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C. RESULTS When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. CONCLUSION The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Cryoletters
2015
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| Subjects: | Boar sperm, Cryopreservation, Freezing extender, PHsperm quality, |
| Online Access: | http://hdl.handle.net/10261/291620 |
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