Immunogenicity and efficacy of a chimpanzee adenovirus-vectored Rift Valley Fever vaccine in mice

Background Rift Valley Fever (RVF) is a viral zoonosis that historically affects livestock production and human health in sub-Saharan Africa, though epizootics have also occurred in the Arabian Peninsula. Whilst an effective live-attenuated vaccine is available for livestock, there is currently no licensed human RVF vaccine. Replication-deficient chimpanzee adenovirus (ChAd) vectors are an ideal platform for development of a human RVF vaccine, given the low prevalence of neutralizing antibodies against them in the human population, and their excellent safety and immunogenicity profile in human clinical trials of vaccines against a wide range of pathogens. Methods. Here, in BALB/c mice, we evaluated the immunogenicity and efficacy of a replication-deficient chimpanzee adenovirus vector, ChAdOx1, encoding the RVF virus envelope glycoproteins, Gn and Gc, which are targets of virus neutralizing antibodies. The ChAdOx1-GnGc vaccine was assessed in comparison to a replication-deficient human adenovirus type 5 vector encoding Gn and Gc (HAdV5-GnGc), a strategy previously shown to confer protective immunity against RVF in mice. Results A single immunization with either of the vaccines conferred protection against RVF virus challenge eight weeks post-immunization. Both vaccines elicited RVF virus neutralizing antibody and a robust CD8§ssup§+§esup§ T cell response. Conclusions Together the results support further development of RVF vaccines based on replication-deficient adenovirus vectors, with ChAdOx1-GnGc being a potential candidate for use in future human clinical trials. © 2013 Warimwe et al.; licensee BioMed Central Ltd.

Bibliographic Details

Main Authors:	Warimwe, G. M., Lorenzo Alguacil, Gema, Lopez-Gil, E., Reyes-Sandoval, A., Cottingham, M. G., Spencer, A. J., Collins, K. A., Dicks, M. D., Milicic, A., Lall, A., Furze, J., Turner, A. V., Hill, A. V., Brun Torres, Alejandro, Gilbert, S. C.
Format:	artículo biblioteca
Language:	English
Published:	BioMed Central 2013
Subjects:	Rift Valley Fever, Adenovirus vector, Vaccine,
Online Access:	http://hdl.handle.net/20.500.12792/4762 http://hdl.handle.net/10261/290413
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