In vitro toxicity of antimicrobials on RTG-2 and RTL-W1 fish cell lines
This study proposed a battery of endpoints based on in vitro fish cell lines. Two fish cell lines and four toxicity endpoints are considered. The tetracycline (TC), oxytetracycline (OTC), sulfachlorpyridazine (SCP), and septrin® (ST) were selected as model antimicrobials and chlorpyrifos as positive control. EROD was induced by septrin® (formulation containing sulfamethoxazole and trimethoprim) at concentrations higher than 20 mg/L, but inhibited by tetracycline, oxytetracycline and chlorpyrifos. Dose dependent inhibition responses were observed for β-galactosidase (β-Gal) activity in cells exposed to septrin®, tetracycline or oxytetracycline. The EROD EC50s ranged between 2.29 × 10-2 mg/L (chlorpyrifos) and 167.63 mg/L (tetracycline). The β-Gal EC50s ranged between 22.1 (septrin®) and 84.59 mg/L (tetracycline). Data suggest that in vitro testing using a battery of endpoints can be a cost-effective solution for screening the toxicity of antimicrobials on fish. The absence of in vitro effects at concentrations well above those expected/measured in the environment may replace the need for conducting acute lethality tests on fish. © 2004 Elsevier B.V. All rights reserved.
| Main Authors: | , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2005
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| Subjects: | Antimicrobials, Chlorpyrifos, RTG-2, RTL-W1, |
| Online Access: | http://hdl.handle.net/20.500.12792/2452 http://hdl.handle.net/10261/290393 |
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