Copper distribution in different proteinic fractions in rainbow trout experimentally poisoned with copper sulfate
Copper acute lethal poisoning in rainbow trout, Oncorhynchus mykiss, does not mean a significative increase of this metal in any specific organ. This fact may be due to the copper high individual variability, in relation with its condition of essential metal. Since copper toxicity involves different cellular proteins, this work tries to elucidate whether experimental exposure would produce any variation in the copper distribution among the different proteinic fractions. Fish exposed to a lethal copper concentration (750 μg Cu/L), were collected just immediately after death. Liver samples were homogenized in saccharose 0,25 M, sonicated for 15 seconds and centrifuged at 18000 x g. Supernatants were filtrated through 0,45 μm and fractioned in an HPLC system. Fractions were analyzed by Atomic Absorption Spectrometry. Different copper distribution between control and exposed fish were observed. The exposure led to an increase of copper concentration in proteins with molecular weights between 10-27 K. Data suggest that these differences could have a potential value to assess acute copper exposure and to diagnose this poisoning.
| Main Authors: | , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Asociación Española de Toxicología
1994
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| Subjects: | Copper, Liver, Proteins, Rainbow trout, |
| Online Access: | http://hdl.handle.net/20.500.12792/5744 http://hdl.handle.net/10261/290005 |
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