Direct PCR detection of foot-and-mouth disease virus

A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR. © 1994.

Bibliographic Details

Main Authors:	Rodríguez, A., Núñez, J. I., Nolasco, G., Ponz Ascaso, Fernando, Sobrino, F., de Blas, C.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 1994
Subjects:	FMDV diagnosis, Direct RT-PCR, Virus detection,
Online Access:	http://hdl.handle.net/20.500.12792/3767 http://hdl.handle.net/10261/290004
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