Light-photoreceptors and proteins related to Monilinia laxa photoresponses

Light represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three <i>Monilinia laxa</i> isolates. The highest mycelial growth rate was observed under red light for the three <i>M. laxa</i> isolates, followed by green light, daylight or darkness. However, reduced sporulation levels were observed in darkness and red light, but conidiation enhancement was found under daylight, black and green light with more hours of exposure to light. Putative photoreceptors for blue (white-collar and cryptochromes), green (opsins), and red light (phytochromes) were identified, and the photoresponse-related regulatory family of velvet proteins. A unique ortholog for each photoreceptor was found, and their respective domain architecture was highly conserved. Transcriptional analyses of uncovered sets of genes were performed under daylight or specific color light, and both in time course illumination, finding light-dependent triggered gene expression of <i>MlVEL2</i>, <i>MlPHY2</i>, <i>MlOPS2</i>, and <i>MlCRY2</i>, and color light as a positive inductor of <i>MlVEL3</i>, <i>MlVEL4</i>, <i>MlPHY1</i>, and <i>MlCRY1</i> expression. <i>M. laxa</i> has a highly conserved set of photoreceptors with other light-responsive fungi. Our phenotypic analyses and the existence of this light-sensing machinery suggest transcriptional regulatory systems dedicated to modulating the development and dispersion of this pathogen.

Bibliographic Details

Main Authors:	Rodríguez-Pires, Silvia, Espeso, Eduardo A., Rasiukevičiūtė, Neringa, Melgarejo, Paloma, Cal, Antonieta de
Other Authors:	European Commission
Format:	artículo biblioteca
Published:	Multidisciplinary Digital Publishing Institute 2021-01-07
Subjects:	Photoreceptors, Light response, Monilinia laxa, Brown rot, Photobiology,
Online Access:	http://hdl.handle.net/10261/227875 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329
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