Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon

There is a high international demand for timber from the genus Dipteryx, or “shihuahuaco” as it is known in Peru. Developing tools that allow the identification and discrimination of Dipteryx species is therefore important for supporting management of natural populations and to underpin legal trade of its timber. The objective of this study was the molecular characterization of Dipteryx species in the Peruvian Amazonia. Two plastid regions (cpDNA: trnH–psbA and matK) were sequenced and 11 microsatellite markers (nDNA) were genotyped for 32 individuals identified as Dipteryx charapilla, D. micrantha morphotype 1 and D. micrantha morphotype 2. Using the concatenated sequences of the plastid genes, we identified ten haplotypes that were not shared between the species or between the D. micrantha morphotypes. Haplotypic diversity was greater in D. micrantha morphotype 2 and D. charapilla than in D. micrantha morphotype 1, which presented only one haplotype with a wide distribution in Peru. The microsatellites allowed the discrimination of the same three clades and identified diagnostic alleles for each clade. These results allowed us to demonstrate that the two morphotypes of D. micrantha are different at both the plastid and nuclear markers, which supports the existence of three genetically distinct species in Peru. This study provides information for the genetic discrimination of Dipteryx species and emphasises the importance of conserving the genetic variability of this genus in the Peruvian Amazonia.

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Main Authors: García Dávila, Carmen, Aldana Gomero, David, Renno, Jean-François, Díaz Soria, Rossana, Hidalgo Pizango, Gabriel, Flores Llampazo, Gerardo, Castro Ruiz, Diana, Mejía de Loayza, Eduardo, Angulo Chávez, Carlos, Mader, Malte, Tysklind, Niklas, Paredes Villanueva, Kathelyn, Del Castillo Torres, Dennis, Degen, Bernd, Honorio Coronado, Eurídice
Format: info:eu-repo/semantics/article biblioteca
Language:eng
Published: Springer Nature 2019-12
Subjects:Dipteryx charapilla, Dipteryx micrantha, Variación genética, Distancia genética, Microsatélites, Secuencia de ADN, Dipteryx, Genotipos, Loreto, Ucayali, Madre de Dios, Amazonía,
Online Access:https://hdl.handle.net/20.500.12921/418
https://doi.org/10.1007/s10709-019-00082-2
https://doi.org/10.1007/s10709-019-00082-2
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spelling dig-iiap-pe-20.500.12921-4182022-12-30T00:06:12Z Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon García Dávila, Carmen Aldana Gomero, David Renno, Jean-François Díaz Soria, Rossana Hidalgo Pizango, Gabriel Flores Llampazo, Gerardo Castro Ruiz, Diana Mejía de Loayza, Eduardo Angulo Chávez, Carlos Mader, Malte Tysklind, Niklas Paredes Villanueva, Kathelyn Del Castillo Torres, Dennis Degen, Bernd Honorio Coronado, Eurídice Dipteryx charapilla Dipteryx micrantha Variación genética Distancia genética Microsatélites Secuencia de ADN Dipteryx Genotipos Loreto Ucayali Madre de Dios Amazonía There is a high international demand for timber from the genus Dipteryx, or “shihuahuaco” as it is known in Peru. Developing tools that allow the identification and discrimination of Dipteryx species is therefore important for supporting management of natural populations and to underpin legal trade of its timber. The objective of this study was the molecular characterization of Dipteryx species in the Peruvian Amazonia. Two plastid regions (cpDNA: trnH–psbA and matK) were sequenced and 11 microsatellite markers (nDNA) were genotyped for 32 individuals identified as Dipteryx charapilla, D. micrantha morphotype 1 and D. micrantha morphotype 2. Using the concatenated sequences of the plastid genes, we identified ten haplotypes that were not shared between the species or between the D. micrantha morphotypes. Haplotypic diversity was greater in D. micrantha morphotype 2 and D. charapilla than in D. micrantha morphotype 1, which presented only one haplotype with a wide distribution in Peru. The microsatellites allowed the discrimination of the same three clades and identified diagnostic alleles for each clade. These results allowed us to demonstrate that the two morphotypes of D. micrantha are different at both the plastid and nuclear markers, which supports the existence of three genetically distinct species in Peru. This study provides information for the genetic discrimination of Dipteryx species and emphasises the importance of conserving the genetic variability of this genus in the Peruvian Amazonia. Programa Nacional de Innovación para la Competitividad y Productividad Innóvate-Perú (contrato 381-PNICP-PIAP-2014); German Federal Ministry of Food and Agriculture (BMEL, Large Scale Project, Grant 28I-001-01) Revisión por pares. 2019-12-23T14:50:53Z 2019-12-23T14:50:53Z 2019-12 info:eu-repo/semantics/article Genetica (2019): 1-12 1573-6857 https://hdl.handle.net/20.500.12921/418 https://doi.org/10.1007/s10709-019-00082-2 Genetica https://doi.org/10.1007/s10709-019-00082-2 eng info:eu-repo/semantics/article https://link.springer.com/article/10.1007%2Fs10709-019-00082-2 info:eu-repo/semantics/openAccess All Rights Reserved Springer Nature Switzerland AG 2019 application/pdf application/pdf text/plain; charset=utf-8 Springer Nature Instituto de Investigaciones de la Amazonía Peruana Repositorio Institucional - IIAP
institution IIAP PE
collection DSpace
country Perú
countrycode PE
component Bibliográfico
access En linea
databasecode dig-iiap-pe
tag biblioteca
region America del Sur
libraryname Biblioteca del IIAP Perú
language eng
topic Dipteryx charapilla
Dipteryx micrantha
Variación genética
Distancia genética
Microsatélites
Secuencia de ADN
Dipteryx
Genotipos
Loreto
Ucayali
Madre de Dios
Amazonía
Dipteryx charapilla
Dipteryx micrantha
Variación genética
Distancia genética
Microsatélites
Secuencia de ADN
Dipteryx
Genotipos
Loreto
Ucayali
Madre de Dios
Amazonía
spellingShingle Dipteryx charapilla
Dipteryx micrantha
Variación genética
Distancia genética
Microsatélites
Secuencia de ADN
Dipteryx
Genotipos
Loreto
Ucayali
Madre de Dios
Amazonía
Dipteryx charapilla
Dipteryx micrantha
Variación genética
Distancia genética
Microsatélites
Secuencia de ADN
Dipteryx
Genotipos
Loreto
Ucayali
Madre de Dios
Amazonía
García Dávila, Carmen
Aldana Gomero, David
Renno, Jean-François
Díaz Soria, Rossana
Hidalgo Pizango, Gabriel
Flores Llampazo, Gerardo
Castro Ruiz, Diana
Mejía de Loayza, Eduardo
Angulo Chávez, Carlos
Mader, Malte
Tysklind, Niklas
Paredes Villanueva, Kathelyn
Del Castillo Torres, Dennis
Degen, Bernd
Honorio Coronado, Eurídice
Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
description There is a high international demand for timber from the genus Dipteryx, or “shihuahuaco” as it is known in Peru. Developing tools that allow the identification and discrimination of Dipteryx species is therefore important for supporting management of natural populations and to underpin legal trade of its timber. The objective of this study was the molecular characterization of Dipteryx species in the Peruvian Amazonia. Two plastid regions (cpDNA: trnH–psbA and matK) were sequenced and 11 microsatellite markers (nDNA) were genotyped for 32 individuals identified as Dipteryx charapilla, D. micrantha morphotype 1 and D. micrantha morphotype 2. Using the concatenated sequences of the plastid genes, we identified ten haplotypes that were not shared between the species or between the D. micrantha morphotypes. Haplotypic diversity was greater in D. micrantha morphotype 2 and D. charapilla than in D. micrantha morphotype 1, which presented only one haplotype with a wide distribution in Peru. The microsatellites allowed the discrimination of the same three clades and identified diagnostic alleles for each clade. These results allowed us to demonstrate that the two morphotypes of D. micrantha are different at both the plastid and nuclear markers, which supports the existence of three genetically distinct species in Peru. This study provides information for the genetic discrimination of Dipteryx species and emphasises the importance of conserving the genetic variability of this genus in the Peruvian Amazonia.
format info:eu-repo/semantics/article
topic_facet Dipteryx charapilla
Dipteryx micrantha
Variación genética
Distancia genética
Microsatélites
Secuencia de ADN
Dipteryx
Genotipos
Loreto
Ucayali
Madre de Dios
Amazonía
author García Dávila, Carmen
Aldana Gomero, David
Renno, Jean-François
Díaz Soria, Rossana
Hidalgo Pizango, Gabriel
Flores Llampazo, Gerardo
Castro Ruiz, Diana
Mejía de Loayza, Eduardo
Angulo Chávez, Carlos
Mader, Malte
Tysklind, Niklas
Paredes Villanueva, Kathelyn
Del Castillo Torres, Dennis
Degen, Bernd
Honorio Coronado, Eurídice
author_facet García Dávila, Carmen
Aldana Gomero, David
Renno, Jean-François
Díaz Soria, Rossana
Hidalgo Pizango, Gabriel
Flores Llampazo, Gerardo
Castro Ruiz, Diana
Mejía de Loayza, Eduardo
Angulo Chávez, Carlos
Mader, Malte
Tysklind, Niklas
Paredes Villanueva, Kathelyn
Del Castillo Torres, Dennis
Degen, Bernd
Honorio Coronado, Eurídice
author_sort García Dávila, Carmen
title Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
title_short Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
title_full Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
title_fullStr Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
title_full_unstemmed Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon
title_sort molecular evidence for three genetic species of dipteryx in the peruvian amazon
publisher Springer Nature
publishDate 2019-12
url https://hdl.handle.net/20.500.12921/418
https://doi.org/10.1007/s10709-019-00082-2
https://doi.org/10.1007/s10709-019-00082-2
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