Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy
1 página.-- Proceedings of the Canadian Meat Science Association Technical Symposium (Halifax, Canadá, 6-7 octubre de 2011)
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dig-igm-es-10261-794562016-05-30T08:38:43Z Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy Prieto, Nuria Dugan, M. E. R. López Campos, Oscar McAllister, T. A. Aalhus, J. L. Uttaro, B. 1 página.-- Proceedings of the Canadian Meat Science Association Technical Symposium (Halifax, Canadá, 6-7 octubre de 2011) Today, consumers are interested in fat composition as scientific evidence suggests that diets high in saturated fat are associated with an increased risk of cardiovascular disease. Techniques to quantify fatty acids (FA) in meat are costly and time-consuming. Near infrared reflectance spectroscopy (NIRS) is a rapid and non destructive method. The aim of this study was to examine the ability of NIRS to estimate the concentration of polyunsaturated FA and their biohydrogenation products in subcutaneous fat from cows fed flaxseed. Subcutaneous fat samples at the 12th rib of 62 cows were collected and stored at -80ºC. After thawing, 32 spectra from each intact sample were collected, then averaged, over a NIR spectral range from 400 to 2498 nm at 31ºC (warm samples) and 2ºC (cold samples), and then analyzed for FA profiles. NIRS calibrations on warm samples, tested by cross-validation, showed high predictability for most of the n-3 (R2: 0.81- 0.86; RMSECV: 0.11-1.56 mg·g-1 fat) and linolenic acid biohydrogenation products such as conjugated linolenic acids, conjugated linoleic acids (CLA), non-CLA dienes and trans-monounsaturated FA with R2 (RMSECV, mg·g-1 fat) of 0.85-0.85 (0.16-0.37), 0.84-0.90 (0.21-2.58), 0.90 (5.49) and 0.84-0.90 (4.24-8.83), respectively. When spectra were obtained from cold samples NIRS predictability was lower than that for warm samples, probably due to a less homogeneous distribution of fat throughout the cells and more air bubbles, or to reduced molecular vibration due to the cooler temperature. NIRS could discriminate 100% of subcutaneous fat samples from cows fed different diets (with or without flaxseed). These data support that NIRS has the potential to be used as a fast and accurate predictor of content of linolenic acid and its biohydrogenation products in subcutaneous fat of beef cows. Nevertheless, further studies are required to test its ability during on-line operations in an abattoir. Peer reviewed 2013-07-11T11:40:04Z 2013-07-11T11:40:04Z 2011-10 póster de congreso http://purl.org/coar/resource_type/c_6670 Proceedings of the Canadian Meat Science Association Technical Symposium (2011) http://hdl.handle.net/10261/79456 en http://cmc-cvc.com/sites/default/files/files/CMSAabstracts2011.pdf open Canadian Meat Science Association |
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1 página.-- Proceedings of the Canadian Meat Science Association Technical Symposium (Halifax, Canadá, 6-7 octubre de 2011) |
format |
póster de congreso |
author |
Prieto, Nuria Dugan, M. E. R. López Campos, Oscar McAllister, T. A. Aalhus, J. L. Uttaro, B. |
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Prieto, Nuria Dugan, M. E. R. López Campos, Oscar McAllister, T. A. Aalhus, J. L. Uttaro, B. Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
author_facet |
Prieto, Nuria Dugan, M. E. R. López Campos, Oscar McAllister, T. A. Aalhus, J. L. Uttaro, B. |
author_sort |
Prieto, Nuria |
title |
Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
title_short |
Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
title_full |
Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
title_fullStr |
Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
title_full_unstemmed |
Predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
title_sort |
predicting the content of polyunsaturated fatty acids and biohydrogenation products in subcutaneous fat of beef cows fed flaxseed by near infrared reflectance spectroscopy |
publisher |
Canadian Meat Science Association |
publishDate |
2011-10 |
url |
http://hdl.handle.net/10261/79456 |
work_keys_str_mv |
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