Screening anthelmintic resistance to triclabendazole in Fasciola hepatica isolated from sheep by means of an egg hatch assay
[Background] In the present study, the detection of anthelmintic resistance to triclabendazole (TCBZ) in sheep infected by Fasciola hepatica was studied using an egg hatch assay (EHA). F. hepatica eggs were recovered from bile and faeces of infected animals by isolates with different grade of anthelmintic resistance to TCBZ: i) a resistant isolate (RT); ii) a susceptible isolate (ST); iii) naturally infected sheep by a susceptible field strain (FST). In the EHA the percentage of hatched eggs were calculated according to the following concentrations of TCBZ diluted in dimethyl-sulfoxide (DMSO): 0.05, 0.2, 1, 5, and 25 μg/ml.
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| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
BioMed Central
2015-08-28
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| Subjects: | Fasciola hepatica, Anthelmintic resistance, Egg hatch assay, Triclabendazole, |
| Online Access: | http://hdl.handle.net/10261/125885 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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