Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy
Trace elements are essential for the normal spermatogenesis of mammals and play a critical role in sperm quality and pathological processes e.g. inflammation. Consequently, multi-elemental analysis of seminal plasma (SP) may provide significant information on physiological and pathophysiological processes occurring in the male reproductive tract. Therefore, the development of a simple, fast and reliable method for seminal plasma (SP) analysis by total reflection X-ray fluorescence spectrometry (TXRF) could be useful for both, scientific and clinical studies. In this study, a detailed assessment of the sample preparation parameters and measurement conditions, including analysis ofthe shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry, was carried out. Using the best analytical conditions, limits of detection for trace elements were found to be in the range of 0.04-0.3 mg kg-1. Trueness and precisionof the results, evaluated by spiked SP sample analysis, were in most cases acceptable with recoveryvalues in the range of 87-109% and relative standard deviations 3-12% (n = 5). The developed TXRF method was applied for the analysis of several SP samples from patients with different diagnoses andthe results were compared with those obtained by ICP-OES. Among the studied trace elements with a role in the antioxidant defence system only Zn could be quantified and some differences in Zn concentrations among studied groups were observed. However, further studies on a large number of samples are required to define the exact relationship between the element composition and semen quality.
| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Published: |
Royal Society of Chemistry (UK)
2020
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| Online Access: | http://hdl.handle.net/10261/232130 |
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