Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion

Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.

Bibliographic Details

Main Authors:	Faria, Melissa, Prats, Eva, Rosas Ramírez, Jonathan Ricardo, Bellot, Marina, Bedrossiantz, Juliette, Pagano, María, Valls, Arnau, Gómez-Canela, Cristian, Porta, Josep M., Mestres, Jordi, García-Reyero, Natàlia, Faggio, Caterina, Gómez Oliván, Leobardo Manuel, Raldúa, Demetrio
Other Authors:	Raldúa, Demetrio [0000-0001-5256-1641]
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2021-02-15
Subjects:	Neurotoxicity, Predicted target profile, Risk assessment, Acetylcholinesterase inhibitor, Androgenic effects, Encocrine disruptors,
Online Access:	http://hdl.handle.net/10261/229828
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