Isotopic evidence of significant assimilation of atmospheric-derived nitrogen fixed by Azospirillum brasilense co-inoculated with phosphate-solubilising Pantoea dispersa in pepper seedling
The contribution of plant growth-promoting bacteria (PGPB) of the genus Azospirillum to the plant N budget through biological nitrogen fixation (BNF) is still controversial. The aim of this study was to determine the contribution of BNF by Azospirillum brasilense on pepper grown at different N levels, attained using the 15N natural abundance method. To this end, pepper plants were grown in a growth chamber and treated with A. brasilense combined with Pantoea dispersa and then irrigated at four different N levels (0, 1, 3 and 7 mM NO3¿). The assimilation of fixed N was clear from the lower ¿15N values observed in bacteria-treated plants compared with those of non-bacteria treated plants. The percentage of BNF-derived N decreased with decreasing NO3¿ levels in the growth medium. BNF contribution to the total nitrogen content of plants was found to be as high as 46%. The results suggest that the bacteria have a potential to supply a considerable amount of N to pepper seedlings, as well as to stimulate plant growth and N uptake when Azospirillum¿Pantoea treatment is combined with low NO3¿ levels.
| Main Authors: | , , , |
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| Format: | artículo biblioteca |
| Published: |
Elsevier
2010
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| Subjects: | Plant-growth promoting bacteria, Nitrate, Biological nitrogen fixation, 15N, Azospirillum, |
| Online Access: | http://hdl.handle.net/10261/97819 |
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