Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development
Two hydrolysates were obtained from dried Mastocarpus stellatus using alcalase. The phenolic content was partially removed from one of them. The phenolic-partially-removed hydrolysate (H) was found to be a potent angiotensin-I converting enzyme (ACE) inhibitor. However, the phenolic-containing hydrolysate (Hp) showed a higher Folin-reactive substance content and antioxidant capacity (reducing power and radical scavenging capacity). Hp was therefore selected for the development of antioxidant Mastocarpus carrageenan-based films. F-Hp0 (without the hydrolysate), F-Hp15 (with 15% hydrolysate) and F-Hp30 (with 30% hydrolysate) films were developed. κ/ι-hybrid carrageenan was the main film constituent and hydrolysate addition resulted in an increased sulfated proportion, higher protein content and higher number of hydrogen bonds. Therefore interactions between carrageenan helices, plasticizers and peptides in the film-forming solution were enhanced, especially in F-Hp15, and consequently the water vapour permeability (WVP) of the resulting film decreased. Nevertheless, F-Hp30 considerably improved the transparency, UV/Vis light barrier, water resistance and elongation at break (EAB). The presence of Hp increased both puncture force (F) and puncture elongation (E), but not tensile strength (TS) or Young's modulus (Y). The addition of an increased concentration of hydrolysate to the films led to a considerable increase in the Folin-reactive substance content and the antioxidant activity, especially the radical scavenging capacity. © 2014 The Royal Society of Chemistry.
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2014
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dig-ictan-es-10261-929192020-01-28T08:26:58Z Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development Blanco Pascual, Nuria Alemán, Ailén Gómez Guillén, M. C. Montero García, Pilar Two hydrolysates were obtained from dried Mastocarpus stellatus using alcalase. The phenolic content was partially removed from one of them. The phenolic-partially-removed hydrolysate (H) was found to be a potent angiotensin-I converting enzyme (ACE) inhibitor. However, the phenolic-containing hydrolysate (Hp) showed a higher Folin-reactive substance content and antioxidant capacity (reducing power and radical scavenging capacity). Hp was therefore selected for the development of antioxidant Mastocarpus carrageenan-based films. F-Hp0 (without the hydrolysate), F-Hp15 (with 15% hydrolysate) and F-Hp30 (with 30% hydrolysate) films were developed. κ/ι-hybrid carrageenan was the main film constituent and hydrolysate addition resulted in an increased sulfated proportion, higher protein content and higher number of hydrogen bonds. Therefore interactions between carrageenan helices, plasticizers and peptides in the film-forming solution were enhanced, especially in F-Hp15, and consequently the water vapour permeability (WVP) of the resulting film decreased. Nevertheless, F-Hp30 considerably improved the transparency, UV/Vis light barrier, water resistance and elongation at break (EAB). The presence of Hp increased both puncture force (F) and puncture elongation (E), but not tensile strength (TS) or Young's modulus (Y). The addition of an increased concentration of hydrolysate to the films led to a considerable increase in the Folin-reactive substance content and the antioxidant activity, especially the radical scavenging capacity. © 2014 The Royal Society of Chemistry. This research was supported by the Spanish Ministry of Science and Innovation, I+D+I National Plan, under the project AGL2011-27607 and the Xunta de Galicia Sectorial programme PEME I+D E I+D SUMA Peer Reviewed 2014-03-04T11:53:06Z 2014-03-04T11:53:06Z 2014 2014-03-04T11:53:06Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1039/c3fo60310e issn: 2042-6496 Food and Function 5: 319- 329 (2014) http://hdl.handle.net/10261/92919 10.1039/c3fo60310e open Royal Society of Chemistry (UK) |
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Two hydrolysates were obtained from dried Mastocarpus stellatus using alcalase. The phenolic content was partially removed from one of them. The phenolic-partially-removed hydrolysate (H) was found to be a potent angiotensin-I converting enzyme (ACE) inhibitor. However, the phenolic-containing hydrolysate (Hp) showed a higher Folin-reactive substance content and antioxidant capacity (reducing power and radical scavenging capacity). Hp was therefore selected for the development of antioxidant Mastocarpus carrageenan-based films. F-Hp0 (without the hydrolysate), F-Hp15 (with 15% hydrolysate) and F-Hp30 (with 30% hydrolysate) films were developed. κ/ι-hybrid carrageenan was the main film constituent and hydrolysate addition resulted in an increased sulfated proportion, higher protein content and higher number of hydrogen bonds. Therefore interactions between carrageenan helices, plasticizers and peptides in the film-forming solution were enhanced, especially in F-Hp15, and consequently the water vapour permeability (WVP) of the resulting film decreased. Nevertheless, F-Hp30 considerably improved the transparency, UV/Vis light barrier, water resistance and elongation at break (EAB). The presence of Hp increased both puncture force (F) and puncture elongation (E), but not tensile strength (TS) or Young's modulus (Y). The addition of an increased concentration of hydrolysate to the films led to a considerable increase in the Folin-reactive substance content and the antioxidant activity, especially the radical scavenging capacity. © 2014 The Royal Society of Chemistry. |
format |
artículo |
author |
Blanco Pascual, Nuria Alemán, Ailén Gómez Guillén, M. C. Montero García, Pilar |
spellingShingle |
Blanco Pascual, Nuria Alemán, Ailén Gómez Guillén, M. C. Montero García, Pilar Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
author_facet |
Blanco Pascual, Nuria Alemán, Ailén Gómez Guillén, M. C. Montero García, Pilar |
author_sort |
Blanco Pascual, Nuria |
title |
Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
title_short |
Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
title_full |
Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
title_fullStr |
Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
title_full_unstemmed |
Enzyme-assisted extraction of κ/ι-hybrid carrageenan from Mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
title_sort |
enzyme-assisted extraction of κ/ι-hybrid carrageenan from mastocarpus stellatus for obtaining bioactive ingredients and their application for edible active film development |
publisher |
Royal Society of Chemistry (UK) |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/92919 |
work_keys_str_mv |
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