Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress
Soluble melanoidins from biscuits were enzymatically solubilized and isolated by sequential ultrafiltration and separated by molecular mass in three different fractions, below 3 kDa, between 3 and 10 kDa, and over 10 kDa; the latter was subsequently digested by simulating gastric plus pancreatic digestive conditions. The four fractions were investigated for their protective effect against an oxidative challenge in HepG2 cells. Pretreatment of cells for 20 h with 0.5-10 μg/mL of any of the four fractions prevented the increased cell damage evoked by the challenge but, except for the intermediate size fraction, did not suppress the increased reactive oxygen species. Antioxidant defenses were rapidly restored after the challenge, and the increase of the oxidative stress biomarker malondialdehyde was prevented by the pretreatment with all but the undigested high molecular mass fraction. The results show that treatment of HepG2 cells with concentrations of biscuit melanoidins within the expected physiological range confers on the cells a significant protection against an oxidative challenge.
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American Chemical Society
2009
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Online Access: | http://hdl.handle.net/10261/77691 |
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dig-ictan-es-10261-776912022-01-26T09:09:47Z Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress Martín, M. Ángeles Ramos, Sonia Mateos, Raquel Rufián Henares, J. A. Morales, F. J. Bravo, Laura Goya, Luis Soluble melanoidins from biscuits were enzymatically solubilized and isolated by sequential ultrafiltration and separated by molecular mass in three different fractions, below 3 kDa, between 3 and 10 kDa, and over 10 kDa; the latter was subsequently digested by simulating gastric plus pancreatic digestive conditions. The four fractions were investigated for their protective effect against an oxidative challenge in HepG2 cells. Pretreatment of cells for 20 h with 0.5-10 μg/mL of any of the four fractions prevented the increased cell damage evoked by the challenge but, except for the intermediate size fraction, did not suppress the increased reactive oxygen species. Antioxidant defenses were rapidly restored after the challenge, and the increase of the oxidative stress biomarker malondialdehyde was prevented by the pretreatment with all but the undigested high molecular mass fraction. The results show that treatment of HepG2 cells with concentrations of biscuit melanoidins within the expected physiological range confers on the cells a significant protection against an oxidative challenge. Peer Reviewed 2013-06-06T09:19:47Z 2013-06-06T09:19:47Z 2009 2013-06-06T09:19:47Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1021/jf9006032 issn: 0021-8561 Journal of Agricultural and Food Chemistry 57(16): 7250-7258 (2009) http://hdl.handle.net/10261/77691 10.1021/jf9006032 en none American Chemical Society |
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Soluble melanoidins from biscuits were enzymatically solubilized and isolated by sequential ultrafiltration and separated by molecular mass in three different fractions, below 3 kDa, between 3 and 10 kDa, and over 10 kDa; the latter was subsequently digested by simulating gastric plus pancreatic digestive conditions. The four fractions were investigated for their protective effect against an oxidative challenge in HepG2 cells. Pretreatment of cells for 20 h with 0.5-10 μg/mL of any of the four fractions prevented the increased cell damage evoked by the challenge but, except for the intermediate size fraction, did not suppress the increased reactive oxygen species. Antioxidant defenses were rapidly restored after the challenge, and the increase of the oxidative stress biomarker malondialdehyde was prevented by the pretreatment with all but the undigested high molecular mass fraction. The results show that treatment of HepG2 cells with concentrations of biscuit melanoidins within the expected physiological range confers on the cells a significant protection against an oxidative challenge. |
format |
artículo |
author |
Martín, M. Ángeles Ramos, Sonia Mateos, Raquel Rufián Henares, J. A. Morales, F. J. Bravo, Laura Goya, Luis |
spellingShingle |
Martín, M. Ángeles Ramos, Sonia Mateos, Raquel Rufián Henares, J. A. Morales, F. J. Bravo, Laura Goya, Luis Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
author_facet |
Martín, M. Ángeles Ramos, Sonia Mateos, Raquel Rufián Henares, J. A. Morales, F. J. Bravo, Laura Goya, Luis |
author_sort |
Martín, M. Ángeles |
title |
Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
title_short |
Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
title_full |
Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
title_fullStr |
Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
title_full_unstemmed |
Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress |
title_sort |
biscuit melanoidins of different molecular masses protect human hepg2 cells against oxidative stress |
publisher |
American Chemical Society |
publishDate |
2009 |
url |
http://hdl.handle.net/10261/77691 |
work_keys_str_mv |
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1777670419547947008 |