Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides

Vilcacundo, Rubén; Martínez Villaluenga, Cristina; Miralles, Beatriz; Hernández-Ledesma, Blanca; Ministerio de Economía y Competitividad (España)

Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides

Póster presentado al 3er Congreso Internacional de Alimentos Funcionales y Nutracéuticos: "Diseño y desarrollo de alimentos funcionales y nutracéuticos para la nutrición humana", celebrado en Mazatlán, Sinaloa (México) del 20 al 22 de junio de 2018.

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Bibliographic Details
Main Authors:	Vilcacundo, Rubén, Martínez Villaluenga, Cristina, Miralles, Beatriz, Hernández-Ledesma, Blanca
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	póster de congreso biblioteca
Published:	2018
Subjects:	Kiwicha, Simulated gastrointestinal digestion, Multifunctional peptides,
Online Access:	http://hdl.handle.net/10261/171653 http://dx.doi.org/10.13039/501100003329
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id	dig-ictan-es-10261-171653
record_format	koha
spelling	dig-ictan-es-10261-1716532018-11-30T01:56:21Z Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides Vilcacundo, Rubén Martínez Villaluenga, Cristina Miralles, Beatriz Hernández-Ledesma, Blanca Ministerio de Economía y Competitividad (España) Universidad Técnica de Ambato Kiwicha Simulated gastrointestinal digestion Multifunctional peptides Póster presentado al 3er Congreso Internacional de Alimentos Funcionales y Nutracéuticos: "Diseño y desarrollo de alimentos funcionales y nutracéuticos para la nutrición humana", celebrado en Mazatlán, Sinaloa (México) del 20 al 22 de junio de 2018. [Introduction]: The multifactorial origin of many chronic diseases provides a new framework for the development of multifunctional foods. Bioactive peptides have become very popular because of their demonstrated multiple activities. No data on the potential role of Amaranthus caudatus (kiwicha) proteins as source of bioactive peptides have been reported. [Methods]: A kiwicha protein concentrate was characterized by gel electrophoresis (SDS-PAGE) and subjected to gastrointestinal digestion simulating physiological conditions. The gastric and gastroduodenal digests and the peptidic fractions lower and higher than 5 kDa were analyzed by their antioxidant, angiotensin I-converting enzyme (ACE), dipeptidyl peptidase IV (DPP-IV), α-amylase, and colon cancer cells viability inhibitory activities. Peptides contained in the most active digest were identified by liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). [Results]: The sequential incubation with pepsin and pancreatin resulted in the total degradation of kiwicha proteins. Gastric digest showed higher ACE inhibitory activity while 60 minutes gastroduodenal digest showed the highest antioxidant, dipeptidyl peptidase IV (DPP-IV), α-amylase, and Caco-2 cell viability inhibitory activities. Peptides > 5 kDa were more effective inhibiting colon cancer 32 cell viability whereas peptides < 5 kDa were mainly responsible for the antioxidant, ACE, DPP-IV, and α-amylase inhibitory activities. Thirteen peptides were identified in the most active gastroduodenal digest. Among them, and according to their structure, sequences FLISCLL, SVFDEELS, and DFIILE, might be responsible for the multifunctional effects observed. [Conclusions]: The role of kiwicha proteins as source of multifunctional peptides released under in vitro conditions simulating gastrointestinal digestion is confirmed. Thus, these proteins might be used as ingredients for functional foods to prevent and/or manage chronic diseases related to oxidative stress, hypertension and/or diabetes. This work has received financial support from projects 1373-CPU-P-2014 (Universidad Técnica de Ambato, UTA) and AGL2013-43247-R and AGL2015-66886-R (MINECO, Spain) Peer Reviewed 2018-10-26T12:42:43Z 2018-10-26T12:42:43Z 2018 2018-10-26T12:42:44Z póster de congreso http://purl.org/coar/resource_type/c_6670 3er Congreso Internacional de Alimentos Funcionales y Nutracéuticos (2018) http://hdl.handle.net/10261/171653 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-43247-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-66886-R Publisher's version Sí open
institution	ICTAN ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-ictan-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICTAN España
topic	Kiwicha Simulated gastrointestinal digestion Multifunctional peptides Kiwicha Simulated gastrointestinal digestion Multifunctional peptides
spellingShingle	Kiwicha Simulated gastrointestinal digestion Multifunctional peptides Kiwicha Simulated gastrointestinal digestion Multifunctional peptides Vilcacundo, Rubén Martínez Villaluenga, Cristina Miralles, Beatriz Hernández-Ledesma, Blanca Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
description	Póster presentado al 3er Congreso Internacional de Alimentos Funcionales y Nutracéuticos: "Diseño y desarrollo de alimentos funcionales y nutracéuticos para la nutrición humana", celebrado en Mazatlán, Sinaloa (México) del 20 al 22 de junio de 2018.
author2	Ministerio de Economía y Competitividad (España)
author_facet	Ministerio de Economía y Competitividad (España) Vilcacundo, Rubén Martínez Villaluenga, Cristina Miralles, Beatriz Hernández-Ledesma, Blanca
format	póster de congreso
topic_facet	Kiwicha Simulated gastrointestinal digestion Multifunctional peptides
author	Vilcacundo, Rubén Martínez Villaluenga, Cristina Miralles, Beatriz Hernández-Ledesma, Blanca
author_sort	Vilcacundo, Rubén
title	Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
title_short	Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
title_full	Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
title_fullStr	Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
title_full_unstemmed	Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
title_sort	role of amaranthus caudatus (kiwicha) protein as source of multifunctional peptides
publishDate	2018
url	http://hdl.handle.net/10261/171653 http://dx.doi.org/10.13039/501100003329
work_keys_str_mv	AT vilcacundoruben roleofamaranthuscaudatuskiwichaproteinassourceofmultifunctionalpeptides AT martinezvillaluengacristina roleofamaranthuscaudatuskiwichaproteinassourceofmultifunctionalpeptides AT mirallesbeatriz roleofamaranthuscaudatuskiwichaproteinassourceofmultifunctionalpeptides AT hernandezledesmablanca roleofamaranthuscaudatuskiwichaproteinassourceofmultifunctionalpeptides
_version_	1777670586779041792

Role of Amaranthus caudatus (kiwicha) protein as source of multifunctional peptides

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