Characterization of a halotolerant lipase from the lactic acid bacteria Lactobacillus plantarum useful in food fermentations
© 2014 Elsevier Ltd. Lactobacillus plantarum is a lactic acid bacteria found in fermented foods on which high salt concentrations are present. So far, no salt-tolerant lipase has been described from lactic acid bacteria. In the present study, the gene lp_3562 encoding a putative esterase/lipase was cloned and expressed in Escherichia coli BL21 (DE3) and the overproduced Lp_3562 protein has been biochemically characterized. Lp_3562 is a lipase active on tributyrin and also on other long chain substrates. Although the highest activity was observed at pH 7, the enzyme showed activity at pH 5.0-8.0. Lp_3562 exhibited optimal lipase activity at 40°C and, showing more than 40% of maximal activity at refrigeration temperature (5°C). Conversely, Lp_3562 presented good thermostability exhibiting more than 80% of its maximal activity after 20h incubation at 45°C. More interestingly, Lp_3562 was active under NaCl concentrations higher than 20%. The biochemical properties exhibited by Lp_3562 make this halotolerant lipase attractive for its use in food fermentations.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier
2015-01
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| Subjects: | Tributyrin, Halophilic, Lactic acid bacteria, Food fermentation, Esterase, |
| Online Access: | http://hdl.handle.net/10261/110800 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/100012818 |
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