Hypergravity effects on early development and gene expression in zebrafish (Danio rerio).
Gravity is constant across the entire Earth and thus living organisms are adapted to this force. However, gravity is not the same beyond Earth. The expanding interest in spatial exploration has captured attention in studying biological changes that occurred by gravity in animal and human bodies. However, physiological alterations in animal development and gene molecular pathways altered by gravity are not fully understood. The aim of this work is to increase comprehension of the effects of hypergravity on early development and gene expression in zebrafish (Danio rerio), a popular worldwide animal model. First, a homemade centrifuge with a large diameter and with a speed of 100 rpm (~3 g) was built. Next, zebrafish embryos were subjected to simulated hypergravity (SHG) during 6 days post fertilization (dpf). Every day, survival, hatching rate, and teratology was recorded. At the end of the treatment, zebrafish larvae were collected and gene expression for a battery of genes related to stress, growth, epigenetics, and immune system was studied. Results showed alterations in swimming, flotation, and tail shape but not in hatching rate and fish survival. Current analysis in gene expression has shown a significant upregulation of dnmt3, a gene involved in the novo DNA methylation synthesis. Taken together, these findings shed some light in (epi)genetic-gravity interactions and might help to elucidate gravity effects in living organisms on space
| Main Authors: | , , , |
|---|---|
| Format: | comunicación de congreso biblioteca |
| Published: |
2021-07-05
|
| Online Access: | http://hdl.handle.net/10261/274892 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|