Nitrogen requirements of commercial wine yeast strains during fermentation of a synthetic grape must

Nitrogen deficiencies in grape musts are one of the main causes of stuck or sluggish wine fermentations. Currently, the most common method for dealing with nitrogen-deficient fermentations is adding supplementary nitrogen (usually ammonium phosphate). However, it is important to know the specific nitrogen requirement of each strain, to avoid excessive addition that can lead to microbial instability and ethyl carbamate accumulation. In this study, we aimed to determine the effect of increasing nitrogen concentrations of three different nitrogen sources on growth and fermentation performance in four industrial wine yeast strains. This task was carried out using statistical modeling techniques. The strains PDM and RVA showed higher growth-rate and maximum population size and consumed nitrogen much more quickly than strains ARM and TTA. Likewise, the strains PDM and RVA were also the greatest nitrogen demanders. Thus, we can conclude that these differences in nitrogen demand positively correlated with higher growth rate and higher nitrogen uptake rate. The most direct effect of employing an adequate nitrogen concentration is the increase in biomass, which involves a higher fermentation rate. However, the impact of nitrogen on fermentation rate is not exclusively due to the increase in biomass because the strain TTA, which showed the worst growth behavior, had the best fermentation activity. Some strains may adapt a strategy whereby fewer cells with higher metabolic activity are produced. Regarding the nitrogen source used, all the strains showed the better and worse fermentation performance with arginine and ammonium, respectively. © 2012 Elsevier Ltd.

Bibliographic Details

Main Authors:	Gutiérrez Linares, Alicia, Chiva, Rosana, Sancho, Marta, Beltrán, Gemma, Arroyo López, Francisco Noé, Guillamón, José Manuel
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2012-08
Subjects:	Yeast assimilable nitrogen, Saccharomyces, Cell growth, Fermentation rate, Alcoholic fermentation, Microbial modeling,
Online Access:	http://hdl.handle.net/10261/52879
Tags:	Add Tag No Tags, Be the first to tag this record!