SARS-CoV-2 Detection and Genome Sequencing in Urban Wastewaters
Due to the excretion of SARS-CoV-2 in faeces, the use of wastewater-based epidemiology (WBE) is a useful tool for virus surveillance in large populations. The analysis of this virus includes a concentration step prior to virus detection by RT-qPCR. In addition, the use of massive sequencing allows the detection of specific mutations of clinical importance, as well as the detection of the introduction of new lineages in a specific population. In this chapter, we describe the analysis of SARS-CoV-2 in urban wastewater by the concentration of the samples by precipitation with aluminum chloride, the detection, and quantification of SARS-CoV-2 RNA by RT-qPCR and the genomic sequencing using two different sequencing platforms.
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| Main Authors: | , , |
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| Other Authors: | |
| Format: | capítulo de libro biblioteca |
| Language: | English |
| Published: |
Springer
2024
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| Subjects: | Genome sequencing, Illumina sequencing, Nanopore sequencing, RT-qPCR, SARS-CoV-2, Wastewater, |
| Online Access: | http://hdl.handle.net/10261/360213 https://api.elsevier.com/content/abstract/scopus_id/85179017197 |
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