Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing
Testes are very prone to be damaged by environmental pollutants, but there is a lack of information about the impact of "chemical cocktails" (CC) on the testicular metabolome and the possible influence in the gut-gonad crosstalk. For this, BALB/c mice were given flumequine and diclofenac orally in food and potentially toxic trace elements (Cd, Hg, As) in drinking water. A mice group was supplemented with selenium, a well-known antagonist against many pollutants. Our results revealed that the steroid 5-alpha-androstan-17-beta-ol propionate, suggested as a parameter of androgenicity independent of testosterone levels, proline that improves reproductive indicators in male rabbits affected by environmental stress) among others metabolites are only present after CC exposure with rodent and selenium supplemented diet. Selenium also antagonized the up-or down-regulation of anandamide (20:l, n-9) (p < 0.001 and FC 0.54 of CC vs C but p > 0,05 and FC 0.74 of CC-Se vs C), that regulates gonadotropin-releasing hormones in mammals, 2,3-dinor-11b-PGF2a (p < 0.001 and FC 0.12 of CC vs C but p > 0,05 and FC 0.34 of CC-Se vs C), which has been related with reproductive hormones, besides others testicular metabolites altered by the exposure to the CC and reversed the levels to control. Moreover, numerous significant associations between gut microbes and testicular metabolites indicated a possible impact of pollutants in the testes mediated by gut microbiota due to a gut-gonad crosstalk.
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Elsevier
2024-03-30
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Subjects: | Chemical cocktails, Metallomics, Microbiota, Selenium, Testicular metabolomics, Ensure healthy lives and promote well-being for all at all ages, testes, microbiomes, selenium, chemical pollutants, metabolomics, intestines, |
Online Access: | http://hdl.handle.net/10261/354528 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100005604 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85190281713 |
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Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines |
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Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines Parra Martínez, Cecilio Selma Royo, Marta Callejón Leblic, Belén Collado, María Carmen Abril, Nieves García-Barrera, Tamara Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
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Testes are very prone to be damaged by environmental pollutants, but there is a lack of information about the impact of "chemical cocktails" (CC) on the testicular metabolome and the possible influence in the gut-gonad crosstalk. For this, BALB/c mice were given flumequine and diclofenac orally in food and potentially toxic trace elements (Cd, Hg, As) in drinking water. A mice group was supplemented with selenium, a well-known antagonist against many pollutants. Our results revealed that the steroid 5-alpha-androstan-17-beta-ol propionate, suggested as a parameter of androgenicity independent of testosterone levels, proline that improves reproductive indicators in male rabbits affected by environmental stress) among others metabolites are only present after CC exposure with rodent and selenium supplemented diet. Selenium also antagonized the up-or down-regulation of anandamide (20:l, n-9) (p < 0.001 and FC 0.54 of CC vs C but p > 0,05 and FC 0.74 of CC-Se vs C), that regulates gonadotropin-releasing hormones in mammals, 2,3-dinor-11b-PGF2a (p < 0.001 and FC 0.12 of CC vs C but p > 0,05 and FC 0.34 of CC-Se vs C), which has been related with reproductive hormones, besides others testicular metabolites altered by the exposure to the CC and reversed the levels to control. Moreover, numerous significant associations between gut microbes and testicular metabolites indicated a possible impact of pollutants in the testes mediated by gut microbiota due to a gut-gonad crosstalk. |
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Agencia Estatal de Investigación (España) |
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Agencia Estatal de Investigación (España) Parra Martínez, Cecilio Selma Royo, Marta Callejón Leblic, Belén Collado, María Carmen Abril, Nieves García-Barrera, Tamara |
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Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines |
author |
Parra Martínez, Cecilio Selma Royo, Marta Callejón Leblic, Belén Collado, María Carmen Abril, Nieves García-Barrera, Tamara |
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Parra Martínez, Cecilio |
title |
Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
title_short |
Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
title_full |
Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
title_fullStr |
Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
title_full_unstemmed |
Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
title_sort |
gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing |
publisher |
Elsevier |
publishDate |
2024-03-30 |
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http://hdl.handle.net/10261/354528 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100005604 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85190281713 |
work_keys_str_mv |
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dig-iata-es-10261-3545282024-05-14T20:46:18Z Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing Parra Martínez, Cecilio Selma Royo, Marta Callejón Leblic, Belén Collado, María Carmen Abril, Nieves García-Barrera, Tamara Agencia Estatal de Investigación (España) European Commission Junta de Andalucía Fundación Ramón Areces Universidad de Huelva 0000-0003-4045-4472 0000-0002-4258-947X #NODATA# #NODATA# 0000-0001-8248-8561 #NODATA# Chemical cocktails Metallomics Microbiota Selenium Testicular metabolomics Ensure healthy lives and promote well-being for all at all ages testes microbiomes selenium chemical pollutants metabolomics intestines Testes are very prone to be damaged by environmental pollutants, but there is a lack of information about the impact of "chemical cocktails" (CC) on the testicular metabolome and the possible influence in the gut-gonad crosstalk. For this, BALB/c mice were given flumequine and diclofenac orally in food and potentially toxic trace elements (Cd, Hg, As) in drinking water. A mice group was supplemented with selenium, a well-known antagonist against many pollutants. Our results revealed that the steroid 5-alpha-androstan-17-beta-ol propionate, suggested as a parameter of androgenicity independent of testosterone levels, proline that improves reproductive indicators in male rabbits affected by environmental stress) among others metabolites are only present after CC exposure with rodent and selenium supplemented diet. Selenium also antagonized the up-or down-regulation of anandamide (20:l, n-9) (p < 0.001 and FC 0.54 of CC vs C but p > 0,05 and FC 0.74 of CC-Se vs C), that regulates gonadotropin-releasing hormones in mammals, 2,3-dinor-11b-PGF2a (p < 0.001 and FC 0.12 of CC vs C but p > 0,05 and FC 0.34 of CC-Se vs C), which has been related with reproductive hormones, besides others testicular metabolites altered by the exposure to the CC and reversed the levels to control. Moreover, numerous significant associations between gut microbes and testicular metabolites indicated a possible impact of pollutants in the testes mediated by gut microbiota due to a gut-gonad crosstalk. This work was supported by the projects: PID2021-123073NB-C21 from the Spanish Ministry of Science and Innovation (MICIN). Generación del Conocimiento. MCI/AEI/FEDER “Una manera de hacer Europa”; UHU-1256905 and UHU-202009 from the FEDER Andalusian Operative Program 2014–2020 (Ministry of Economy, Knowledge, Business and Universities, Regional Government of Andalusia, Spain). The authors are grateful to FEDER (European Community) for financial support, Grant UNHU13-1E-1611. CPM thanks MICIN for a predoctoral grant (ref. PRE2019-091,650). MSR and MCC also acknowledge the award of the Spanish Government MCIN/AEI to the Institute of Agrochemistry and Food Technology (IATA-CSIC) as Centre of Excellence Severo Ochoa (CEX2021-001189-S MCIN/AEI / 10.13039/ 501100011033). The authors would like to acknowledge the support from The Ramón Areces Foundation (ref. CIVP19A5918). Funding for open access charge: Universidad de Huelva/CBUA. With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S) Peer reviewed 2024-04-22T10:27:24Z 2024-04-22T10:27:24Z 2024-03-30 artículo http://purl.org/coar/resource_type/c_6501 Food and Chemical Toxicology 188: 114627 (2024) 0278-6915 CEX2021-001189-S http://hdl.handle.net/10261/354528 10.1016/j.fct.2024.114627 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100005604 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 38561037 2-s2.0-85190281713 https://api.elsevier.com/content/abstract/scopus_id/85190281713 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123073NB-C21/ES/METALOMICA Y METODOLOGIAS (META)OMICAS PARA ESTUDIAR LA NEUROTOXICIDAD Y EFECTOS EN LA SALUD REPRODUCTIVA DE LOS METALES. INFLUENCIA DE LA MICROBIOTA INTESTINAL Y DEL SELENIO/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX 2021-001189-S Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Publisher's version https://doi.org/10.1016/j.fct.2024.114627 Sí open Elsevier |