Gut-gonad crosstalk in mice exposed to a "chemical cocktail" combining metabolomics and microbial profile by amplicon sequencing
Testes are very prone to be damaged by environmental pollutants, but there is a lack of information about the impact of "chemical cocktails" (CC) on the testicular metabolome and the possible influence in the gut-gonad crosstalk. For this, BALB/c mice were given flumequine and diclofenac orally in food and potentially toxic trace elements (Cd, Hg, As) in drinking water. A mice group was supplemented with selenium, a well-known antagonist against many pollutants. Our results revealed that the steroid 5-alpha-androstan-17-beta-ol propionate, suggested as a parameter of androgenicity independent of testosterone levels, proline that improves reproductive indicators in male rabbits affected by environmental stress) among others metabolites are only present after CC exposure with rodent and selenium supplemented diet. Selenium also antagonized the up-or down-regulation of anandamide (20:l, n-9) (p < 0.001 and FC 0.54 of CC vs C but p > 0,05 and FC 0.74 of CC-Se vs C), that regulates gonadotropin-releasing hormones in mammals, 2,3-dinor-11b-PGF2a (p < 0.001 and FC 0.12 of CC vs C but p > 0,05 and FC 0.34 of CC-Se vs C), which has been related with reproductive hormones, besides others testicular metabolites altered by the exposure to the CC and reversed the levels to control. Moreover, numerous significant associations between gut microbes and testicular metabolites indicated a possible impact of pollutants in the testes mediated by gut microbiota due to a gut-gonad crosstalk.
Main Authors: | , , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2024-03-30
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Subjects: | Chemical cocktails, Metallomics, Microbiota, Selenium, Testicular metabolomics, Ensure healthy lives and promote well-being for all at all ages, testes, microbiomes, selenium, chemical pollutants, metabolomics, intestines, |
Online Access: | http://hdl.handle.net/10261/354528 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100005604 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85190281713 |
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Internet
http://hdl.handle.net/10261/354528http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/501100011011
http://dx.doi.org/10.13039/501100005604
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011033
https://api.elsevier.com/content/abstract/scopus_id/85190281713