An approach to As(III) and As(V) bioavailability studies with Caco-2 cells
Foods and drinking water are the main sources of human exposure to inorganic arsenic [As(III) and As(V)]. After oral ingestion, the intestinal epithelium is the first barrier to absorption of these species. A human intestinal cell line (Caco-2) was used to evaluate cell retention and transport of As(III) (15.6-156.0 μM) and/or As(V) (15.4-170.6 μM). Cell monolayer integrity, cell viability, membrane damage and effects on cell metabolism were evaluated. Only the highest concentrations assayed [As(III): 156.0 μM; As(V): 170.6 μM] produced a cytotoxic effect with different cellular targets: As(III) altered the permeability of tight junctions, and As(V) caused uncoupling of the respiratory chain. Retention and transport of As(III) was more efficient than that of As(V). After 4 h of exposure to As(III) or As(V), monolayer retention percentages varied between 0.87-2.28% and 0.14-0.39%, respectively. Transepithelial transport was greater for As(III) (5.82-7.71%) than for As(V) (not detectable-1.55%). The addition of As(III) and As(V) jointly produced a transport rate similar to that observed when they were added independently. © 2005 Elsevier Ltd. All rights reserved.
| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Published: |
Elsevier BV
2005-12
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| Subjects: | Arsenite, Arsenate, Caco-2 cells, Uptake, Transport, Bioavailability, |
| Online Access: | http://hdl.handle.net/10261/331010 |
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