Spatial and temporal distribution of SARS-CoV-2 diversity circulating in wastewater
Wastewater-based epidemiology (WBE) has proven to be an effective tool for epidemiological surveillance of SARS-CoV-2 during the current COVID-19 pandemic. Furthermore, combining WBE together with high-throughput sequencing techniques can be useful for the analysis of SARS-CoV-2 viral diversity present in a given sample. The present study focuses on the genomic analysis of SARS-CoV-2 in 76 sewage samples collected during the three epidemiological waves that occurred in Spain from 14 wastewater treatment plants distributed throughout the country. The results obtained demonstrate that the metagenomic analysis of SARS-CoV-2 in wastewater allows the detection of mutations that define the B.1.1.7 lineage and the ability of the technique to anticipate the detection of certain mutations before they are detected in clinical samples. The study proves the usefulness of sewage sequencing to track Variants of Concern that can complement clinical testing to help in decision-making and in the analysis of the evolution of the pandemic.
Main Authors: | , , , , , , , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2022-03-01
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Subjects: | Genome sequencing, SARS-CoV-2, Spike mutations, Variants of concern, Variants of interest, Wastewater, |
Online Access: | http://hdl.handle.net/10261/264797 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100003359 https://api.elsevier.com/content/abstract/scopus_id/85122669036 |
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Internet
http://hdl.handle.net/10261/264797http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100003359
https://api.elsevier.com/content/abstract/scopus_id/85122669036