Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late)
Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated.
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021-12-01
|
Subjects: | Ascorbic acid, Citrus, Fruit, Leaf, Maturation, Orange, Petal, Vitamin C, |
Online Access: | http://hdl.handle.net/10261/255985 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85119902393 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-iata-es-10261-255985 |
---|---|
record_format |
koha |
spelling |
dig-iata-es-10261-2559852024-05-16T20:46:10Z Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) Alós, Enriqueta Rey, Florencia Gil, José Vicente Rodrigo, María Jesús Zacarías, Lorenzo Generalitat Valenciana European Commission Ministerio de Economía y Competitividad (España) Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated. This work was supported by research grants FP7-PEOPLE-2011-CIG-2011-303652 (Marie Curie Actions, European Union), Proyectos de I+D para Grupos de Investigación Emergentes GV/2012/036 (Generalitat Valenciana, Spain). The financial support of the projects AGL2015-70218 (Ministerio Economía y Competitividad, Spain) and PROMETEOII/2014/027 (Generalitat Valenciana) are also acknowledged. Enriqueta Alós was recipient of a JAE-Doc (CSIC) post-doctoral contract that was co-funded by Fondo Social Europeo de Desarrollo Regional (FEDER). Peer reviewed 2021-12-15T06:56:46Z 2021-12-15T06:56:46Z 2021-12-01 artículo http://purl.org/coar/resource_type/c_6501 Plants 10(12): 2590 (2021) http://hdl.handle.net/10261/255985 10.3390/plants10122590 2223-7747 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 2-s2.0-85119902393 https://api.elsevier.com/content/abstract/scopus_id/85119902393 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70218 Plants Publisher's version https://doi.org/10.3390/plants10122590 Sí open Multidisciplinary Digital Publishing Institute |
institution |
IATA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iata-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IATA España |
language |
English |
topic |
Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C |
spellingShingle |
Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C Alós, Enriqueta Rey, Florencia Gil, José Vicente Rodrigo, María Jesús Zacarías, Lorenzo Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
description |
Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated. |
author2 |
Generalitat Valenciana |
author_facet |
Generalitat Valenciana Alós, Enriqueta Rey, Florencia Gil, José Vicente Rodrigo, María Jesús Zacarías, Lorenzo |
format |
artículo |
topic_facet |
Ascorbic acid Citrus Fruit Leaf Maturation Orange Petal Vitamin C |
author |
Alós, Enriqueta Rey, Florencia Gil, José Vicente Rodrigo, María Jesús Zacarías, Lorenzo |
author_sort |
Alós, Enriqueta |
title |
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
title_short |
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
title_full |
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
title_fullStr |
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
title_full_unstemmed |
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (Citrus sinensis cv. valencia late) |
title_sort |
ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (citrus sinensis cv. valencia late) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021-12-01 |
url |
http://hdl.handle.net/10261/255985 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85119902393 |
work_keys_str_mv |
AT alosenriqueta ascorbicacidcontentandtranscriptionalprofilingofgenesinvolvedinitsmetabolismduringdevelopmentofpetalsleavesandfruitsoforangecitrussinensiscvvalencialate AT reyflorencia ascorbicacidcontentandtranscriptionalprofilingofgenesinvolvedinitsmetabolismduringdevelopmentofpetalsleavesandfruitsoforangecitrussinensiscvvalencialate AT giljosevicente ascorbicacidcontentandtranscriptionalprofilingofgenesinvolvedinitsmetabolismduringdevelopmentofpetalsleavesandfruitsoforangecitrussinensiscvvalencialate AT rodrigomariajesus ascorbicacidcontentandtranscriptionalprofilingofgenesinvolvedinitsmetabolismduringdevelopmentofpetalsleavesandfruitsoforangecitrussinensiscvvalencialate AT zacariaslorenzo ascorbicacidcontentandtranscriptionalprofilingofgenesinvolvedinitsmetabolismduringdevelopmentofpetalsleavesandfruitsoforangecitrussinensiscvvalencialate |
_version_ |
1802820497383096320 |