Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening
An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment.
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Format: | artículo biblioteca |
Language: | English |
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American Chemical Society
2021-06-25
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Subjects: | High-resolution proteomics, Meat biomarkers, Meat quality assessment, Rapid proteomic screening, Pre-slaughter stress, |
Online Access: | http://hdl.handle.net/10261/246695 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003086 http://dx.doi.org/10.13039/100007652 |
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dig-iata-es-10261-2466952022-12-21T13:44:03Z Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening Sentandreu, Enrique Fuente-García, Claudia Pardo, Olga Oliván, Mamen León, Núria Aldai, Noelia Yusà, Vicent Sentandreu, Miguel Ángel Eusko Jaurlaritza Ministerio de Ciencia, Innovación y Universidades (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) European Commission High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment. Department of Economic Development and Infrastructures of the Basque Government is acknowledged for the doctoral fellowship of C.F.G. Funding from Project RTA2014-00034-C04 (Spanish Instituto Nacional de Investigación y Tecnología Agraria, INIA), funding and contract of E. Sentandreu from Project RTI2018-096162-R-C22 (Spanish Agencia Estatal de Investigación), and FEDER funds are also fully acknowledged. Peer reviewed 2021-07-28T06:02:29Z 2021-07-28T06:02:29Z 2021-06-25 artículo http://purl.org/coar/resource_type/c_6501 Journal of Agricultural and Food Chemistry 69 (26): 7478-7487 (2021) 0021-8561 http://hdl.handle.net/10261/246695 10.1021/acs.jafc.1c02016 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003086 http://dx.doi.org/10.13039/100007652 34171191 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2014-00034-C04 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096162-R-C22 Publisher's version https://doi.org/10.1021/acs.jafc.1c02016 Sí open American Chemical Society |
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High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress |
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High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress Sentandreu, Enrique Fuente-García, Claudia Pardo, Olga Oliván, Mamen León, Núria Aldai, Noelia Yusà, Vicent Sentandreu, Miguel Ángel Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
description |
An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment. |
author2 |
Eusko Jaurlaritza |
author_facet |
Eusko Jaurlaritza Sentandreu, Enrique Fuente-García, Claudia Pardo, Olga Oliván, Mamen León, Núria Aldai, Noelia Yusà, Vicent Sentandreu, Miguel Ángel |
format |
artículo |
topic_facet |
High-resolution proteomics Meat biomarkers Meat quality assessment Rapid proteomic screening Pre-slaughter stress |
author |
Sentandreu, Enrique Fuente-García, Claudia Pardo, Olga Oliván, Mamen León, Núria Aldai, Noelia Yusà, Vicent Sentandreu, Miguel Ángel |
author_sort |
Sentandreu, Enrique |
title |
Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
title_short |
Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
title_full |
Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
title_fullStr |
Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
title_full_unstemmed |
Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening |
title_sort |
protein biomarkers of bovine defective meats at a glance: gel-free hybrid quadrupole-orbitrap analysis for rapid screening |
publisher |
American Chemical Society |
publishDate |
2021-06-25 |
url |
http://hdl.handle.net/10261/246695 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003086 http://dx.doi.org/10.13039/100007652 |
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