Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley
Field-grown grapevines are often exposed to multiple environmental stresses, which challenges wine-growers to develop sustainable measures to sustain vine growth, yield, and quality. Under field conditions this task is demanding, due to differences in the magnitudes of stresses and associated plant responses. In this study we explored the hypothesis that kaolin-particle film application improves grapevine photoprotection through the regulation of xanthophyll cycle genes, limiting the thermal dissipation of excess energy under harsh environmental conditions. Hence, we selected two grapevine varieties, Touriga-Nacional (TN) and Touriga-Franca (TF), grown in the Douro Demarcated Region, and evaluated changes in light dissipation mechanisms, xanthophyll cycle components, and the expression of xanthophyll cycle genes during the 2017 summer season. The results showed that, from veraison to ripening, kaolin triggered the up-regulation of violaxanthin de-epoxidase (VvVDE1) and zeaxanthin epoxidase (VvZEP1) genes, indicating optimised regulation of the xanthophyll cycle. Kaolin treatment also decreased chlorophyll (Chla, Chlb, Chl(a+b)) and carotenoid (Car) accumulation under increasing summer stress conditions in both varieties and lowered the non-photochemical quenching (NPQ) of grapevines on ripening, suggesting a long-term response to summer stress. In addition, kaolin-treated grapevines showed increased Chla/Chlb and lower Chl(a+b)/Car ratios, displaying some features of high light adapted leaves. Overall, this study suggests that kaolin application enabled grapevines to benefit from fluctuating periods of summer stress by managing chlorophyll and carotenoid content and limiting down-regulation of both photochemistry and photoinhibition processes. Under Mediterranean field conditions, kaolin application can be considered an efficient method of minimising summer stress impact on grapevines.
Main Authors: | , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2021-03-21
|
Subjects: | Gene expression, Non-photochemical quenching, Photochemistry, Photoprotection, Zeaxanthin, |
Online Access: | http://hdl.handle.net/10261/240111 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-iata-es-10261-240111 |
---|---|
record_format |
koha |
spelling |
dig-iata-es-10261-2401112023-01-03T08:53:21Z Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley Bernardo, S. Dinis, Lia-Tânia Luzio, A. Machado, N. Vives-Peris, V. López-Climent, M.F. Gómez-Cadenas, Aurelio Zacarías, Lorenzo Rodrigo, María Jesús Malheiro, A.C. Correia, C. Moutinho-Pereira, José Fundação para a Ciência e a Tecnologia (Portugal) European Commission Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin Field-grown grapevines are often exposed to multiple environmental stresses, which challenges wine-growers to develop sustainable measures to sustain vine growth, yield, and quality. Under field conditions this task is demanding, due to differences in the magnitudes of stresses and associated plant responses. In this study we explored the hypothesis that kaolin-particle film application improves grapevine photoprotection through the regulation of xanthophyll cycle genes, limiting the thermal dissipation of excess energy under harsh environmental conditions. Hence, we selected two grapevine varieties, Touriga-Nacional (TN) and Touriga-Franca (TF), grown in the Douro Demarcated Region, and evaluated changes in light dissipation mechanisms, xanthophyll cycle components, and the expression of xanthophyll cycle genes during the 2017 summer season. The results showed that, from veraison to ripening, kaolin triggered the up-regulation of violaxanthin de-epoxidase (VvVDE1) and zeaxanthin epoxidase (VvZEP1) genes, indicating optimised regulation of the xanthophyll cycle. Kaolin treatment also decreased chlorophyll (Chla, Chlb, Chl(a+b)) and carotenoid (Car) accumulation under increasing summer stress conditions in both varieties and lowered the non-photochemical quenching (NPQ) of grapevines on ripening, suggesting a long-term response to summer stress. In addition, kaolin-treated grapevines showed increased Chla/Chlb and lower Chl(a+b)/Car ratios, displaying some features of high light adapted leaves. Overall, this study suggests that kaolin application enabled grapevines to benefit from fluctuating periods of summer stress by managing chlorophyll and carotenoid content and limiting down-regulation of both photochemistry and photoinhibition processes. Under Mediterranean field conditions, kaolin application can be considered an efficient method of minimising summer stress impact on grapevines. This work was supported by National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020, the Clim4Vitis project – “Climate change impact mitigation for European viticulture: knowledge transfer for an integrated approach”, funded by the European Union's Horizon 2020 Research and Innovation programme, under grant agreement no. 810176, and by the project I&D&I AgriFood XXI, operation NORTE-01-0145-FEDER-000041, co-funded by the European Regional Development Fund (FEDER) through NORTE 2020 (Programa Operacional Regional do Norte, 2014/2020). Sara Bernardo acknowledges the financial support provided by the FCT-Portuguese Foundation for Science and Technology (PD/BD/128273/2017) under the Doctoral Programme “Agricultural Production Chains – from Fork to Farm”. Peer reviewed 2021-05-07T05:22:11Z 2021-05-07T05:22:11Z 2021-03-21 artículo http://purl.org/coar/resource_type/c_6501 0981-9428 http://hdl.handle.net/10261/240111 10.1016/j.plaphy.2021.03.038 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/810176 Postprint https://doi.org/10.1016/j.plaphy.2021.03.038 Sí open Elsevier |
institution |
IATA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iata-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IATA España |
language |
English |
topic |
Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin |
spellingShingle |
Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin Bernardo, S. Dinis, Lia-Tânia Luzio, A. Machado, N. Vives-Peris, V. López-Climent, M.F. Gómez-Cadenas, Aurelio Zacarías, Lorenzo Rodrigo, María Jesús Malheiro, A.C. Correia, C. Moutinho-Pereira, José Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
description |
Field-grown grapevines are often exposed to multiple environmental stresses, which challenges wine-growers to develop sustainable measures to sustain vine growth, yield, and quality. Under field conditions this task is demanding, due to differences in the magnitudes of stresses and associated plant responses. In this study we explored the hypothesis that kaolin-particle film application improves grapevine photoprotection through the regulation of xanthophyll cycle genes, limiting the thermal dissipation of excess energy under harsh environmental conditions. Hence, we selected two grapevine varieties, Touriga-Nacional (TN) and Touriga-Franca (TF), grown in the Douro Demarcated Region, and evaluated changes in light dissipation mechanisms, xanthophyll cycle components, and the expression of xanthophyll cycle genes during the 2017 summer season. The results showed that, from veraison to ripening, kaolin triggered the up-regulation of violaxanthin de-epoxidase (VvVDE1) and zeaxanthin epoxidase (VvZEP1) genes, indicating optimised regulation of the xanthophyll cycle. Kaolin treatment also decreased chlorophyll (Chla, Chlb, Chl(a+b)) and carotenoid (Car) accumulation under increasing summer stress conditions in both varieties and lowered the non-photochemical quenching (NPQ) of grapevines on ripening, suggesting a long-term response to summer stress. In addition, kaolin-treated grapevines showed increased Chla/Chlb and lower Chl(a+b)/Car ratios, displaying some features of high light adapted leaves. Overall, this study suggests that kaolin application enabled grapevines to benefit from fluctuating periods of summer stress by managing chlorophyll and carotenoid content and limiting down-regulation of both photochemistry and photoinhibition processes. Under Mediterranean field conditions, kaolin application can be considered an efficient method of minimising summer stress impact on grapevines. |
author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
author_facet |
Fundação para a Ciência e a Tecnologia (Portugal) Bernardo, S. Dinis, Lia-Tânia Luzio, A. Machado, N. Vives-Peris, V. López-Climent, M.F. Gómez-Cadenas, Aurelio Zacarías, Lorenzo Rodrigo, María Jesús Malheiro, A.C. Correia, C. Moutinho-Pereira, José |
format |
artículo |
topic_facet |
Gene expression Non-photochemical quenching Photochemistry Photoprotection Zeaxanthin |
author |
Bernardo, S. Dinis, Lia-Tânia Luzio, A. Machado, N. Vives-Peris, V. López-Climent, M.F. Gómez-Cadenas, Aurelio Zacarías, Lorenzo Rodrigo, María Jesús Malheiro, A.C. Correia, C. Moutinho-Pereira, José |
author_sort |
Bernardo, S. |
title |
Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
title_short |
Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
title_full |
Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
title_fullStr |
Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
title_full_unstemmed |
Particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the Douro Valley |
title_sort |
particle film technology modulates xanthophyll cycle and photochemical dynamics of grapevines grown in the douro valley |
publisher |
Elsevier |
publishDate |
2021-03-21 |
url |
http://hdl.handle.net/10261/240111 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100000780 |
work_keys_str_mv |
AT bernardos particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT dinisliatania particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT luzioa particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT machadon particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT vivesperisv particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT lopezclimentmf particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT gomezcadenasaurelio particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT zacariaslorenzo particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT rodrigomariajesus particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT malheiroac particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT correiac particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley AT moutinhopereirajose particlefilmtechnologymodulatesxanthophyllcycleandphotochemicaldynamicsofgrapevinesgrowninthedourovalley |
_version_ |
1777670172986834944 |