Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells

Mandarin juice is a rich source of antioxidant bioactive compounds. While the content and profile of bioactives are known, the impact of high-pressure processing (HPP) on their stability and bioaccessibility (BA) is unknown, but may allow obtaining safe, nutritious, and fresh-tasting juices with highly extractable bioactive compounds. The stability and BA of bioactive antioxidant compounds in untreated and HPP-treated (400 MPa/40 °C/1 min) Clementine mandarin juices, and the cytoprotective effect of its bioaccessible fractions (BF) obtained after simulated gastrointestinal digestion against H2O2-induced oxidative stress in differentiated Caco-2 cells were investigated. The BF of HPP-treated juices showed a better retention of carotenoids, flavonoids, ascorbic acid, total polyphenols and FRAP value, and slightly higher cytoprotection (mitochondrial membrane potential and ROS) than untreated juices. Therefore, HPP can be recommended as a suitable technology to retain or indeed increase antioxidant bioactives and their cytoprotective activity in mandarin juices after gastrointestinal digestion.

Bibliographic Details

Main Authors:	Cilla, Antonio, Rodrigo, María Jesús, Ancos, Begoña de, Sánchez-Moreno, Concepción, Cano, M. Pilar, Zacarías, Lorenzo, Barberá, Reyes, Alegría, Amparo
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	artículo biblioteca
Language:	English
Published:	Royal Society of Chemistry (UK) 2020-09-07
Subjects:	In-vitro bioaccessibility, Pulsed electric-fields, Antioxidant activity, Beta-cryptoxanthin, Ascorbic acid, Orange juice, Vitamin-c, Oxidative stress, Flavonoid composition, Citrus juice,
Online Access:	http://hdl.handle.net/10261/223597 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780
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