Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts
Certain bioactive compounds that derive from tryptophan have been determined in fermented foods, which suggests that the role of yeast is putative in origin. Melatonin is a neurohormone in humans that plays an important role in health. The activity of other compounds, such as 3-indoleacetic acid, has been recently highlighted, and interest in elucidating the conditions of their production has grown. However, the biosynthesis of melatonin by yeasts remains unclear to a large extent. Therefore, this work was undertaken to demonstrate the unequivocal synthesis of melatonin and other compounds that derive from tryptophan metabolism by yeast by determining them in the intracellular compartment. By high-resolution mass spectrometry and a validated method, tryptophan itself, melatonin, serotonin, N-acetyl-5-hydroxytryptamine and 3-indoleacetic acid, were identified in the intracellular compartment of Saccharomyces and non-Saccharomyces wine yeasts.
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Format: | artículo biblioteca |
Language: | English |
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Springer
2019-02-26
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Subjects: | Exact mass, Simultaneous determination, Alcoholic fermentation, Bioactive, 5-Methoxyindoles, Wine yeast, |
Online Access: | http://hdl.handle.net/10261/206075 http://dx.doi.org/10.13039/501100003329 |
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dig-iata-es-10261-2060752020-05-26T08:23:55Z Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts Fernández Cruz, Edwin González, Beatriz Muñiz Calvo, Sara Morcillo Parra, M. Ángeles Bisquert, Ricardo Troncoso, Ana M. García Parrilla, María Carmen Torija, María Jesús Guillamón, José Manuel Ministerio de Economía y Competitividad (España) Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast Certain bioactive compounds that derive from tryptophan have been determined in fermented foods, which suggests that the role of yeast is putative in origin. Melatonin is a neurohormone in humans that plays an important role in health. The activity of other compounds, such as 3-indoleacetic acid, has been recently highlighted, and interest in elucidating the conditions of their production has grown. However, the biosynthesis of melatonin by yeasts remains unclear to a large extent. Therefore, this work was undertaken to demonstrate the unequivocal synthesis of melatonin and other compounds that derive from tryptophan metabolism by yeast by determining them in the intracellular compartment. By high-resolution mass spectrometry and a validated method, tryptophan itself, melatonin, serotonin, N-acetyl-5-hydroxytryptamine and 3-indoleacetic acid, were identified in the intracellular compartment of Saccharomyces and non-Saccharomyces wine yeasts. This work was supported by the Ministry of Economy, Industry and Competitiveness, Spain (Grant no AGL2016-77505-C3). Peer reviewed 2020-04-01T07:22:12Z 2020-04-01T07:22:12Z 2019-02-26 artículo http://purl.org/coar/resource_type/c_6501 European Food Research and Technology 245 (8): 1553-1560 (2019) 1438-2377 http://hdl.handle.net/10261/206075 10.1007/s00217-019-03257-5 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-77505-C3 Postprint https://doi.org/10.1007/s00217-019-03257-5 Sí open Springer |
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Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast |
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Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast Fernández Cruz, Edwin González, Beatriz Muñiz Calvo, Sara Morcillo Parra, M. Ángeles Bisquert, Ricardo Troncoso, Ana M. García Parrilla, María Carmen Torija, María Jesús Guillamón, José Manuel Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
description |
Certain bioactive compounds that derive from tryptophan have been determined in fermented foods, which suggests that the role of yeast is putative in origin. Melatonin is a neurohormone in humans that plays an important role in health. The activity of other compounds, such as 3-indoleacetic acid, has been recently highlighted, and interest in elucidating the conditions of their production has grown. However, the biosynthesis of melatonin by yeasts remains unclear to a large extent. Therefore, this work was undertaken to demonstrate the unequivocal synthesis of melatonin and other compounds that derive from tryptophan metabolism by yeast by determining them in the intracellular compartment. By high-resolution mass spectrometry and a validated method, tryptophan itself, melatonin, serotonin, N-acetyl-5-hydroxytryptamine and 3-indoleacetic acid, were identified in the intracellular compartment of Saccharomyces and non-Saccharomyces wine yeasts. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Fernández Cruz, Edwin González, Beatriz Muñiz Calvo, Sara Morcillo Parra, M. Ángeles Bisquert, Ricardo Troncoso, Ana M. García Parrilla, María Carmen Torija, María Jesús Guillamón, José Manuel |
format |
artículo |
topic_facet |
Exact mass Simultaneous determination Alcoholic fermentation Bioactive 5-Methoxyindoles Wine yeast |
author |
Fernández Cruz, Edwin González, Beatriz Muñiz Calvo, Sara Morcillo Parra, M. Ángeles Bisquert, Ricardo Troncoso, Ana M. García Parrilla, María Carmen Torija, María Jesús Guillamón, José Manuel |
author_sort |
Fernández Cruz, Edwin |
title |
Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
title_short |
Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
title_full |
Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
title_fullStr |
Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
title_full_unstemmed |
Intracellular biosynthesis of melatonin and other indolic compounds in Saccharomyces and non-Saccharomyces wine yeasts |
title_sort |
intracellular biosynthesis of melatonin and other indolic compounds in saccharomyces and non-saccharomyces wine yeasts |
publisher |
Springer |
publishDate |
2019-02-26 |
url |
http://hdl.handle.net/10261/206075 http://dx.doi.org/10.13039/501100003329 |
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