Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins

Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins

Manuscrito correspondiente al artículo publicado con el título "Responses of Saccharomyces cerevisiae Strains from Different Origins to Elevated Iron Concentrations". -- Accepted manuscript posted online 15 January 2016

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Bibliographic Details
Main Authors: Martínez Garay, Carlos Andrés, de Llanos, Rosa, Romero, Antonia, Martínez Pastor, M.Teresa, Puig, Sergi
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: American Society for Microbiology 2016
Subjects:Iron accumulation, Yeast, Oxidative stress, Homeostasis genes,
Online Access:http://hdl.handle.net/10261/131525
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
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spelling dig-iata-es-10261-1315252021-12-27T15:43:11Z Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins Martínez Garay, Carlos Andrés de Llanos, Rosa Romero, Antonia Martínez Pastor, M.Teresa Puig, Sergi Ministerio de Economía y Competitividad (España) Generalitat Valenciana Consejo Superior de Investigaciones Científicas (España) European Commission Iron accumulation Yeast Oxidative stress Homeostasis genes Manuscrito correspondiente al artículo publicado con el título "Responses of Saccharomyces cerevisiae Strains from Different Origins to Elevated Iron Concentrations". -- Accepted manuscript posted online 15 January 2016 Iron is an essential micronutrient for all eukaryotic organisms. However, the low solubility of ferric iron has tremendously increased the prevalence of iron deficiency anemia, especially in women and children, with dramatic consequences. Baker's yeast Saccharomyces cerevisiae is used as a model eukaryotic organism, a fermentative microorganism, and a feed supplement. In this report, we explore the genetic diversity of 123 wild and domestic strains of S. cerevisiae isolated from different geographical origins and sources to characterize how yeast cells respond to elevated iron concentrations in the environment. By using two different forms of iron, we selected and characterized both iron-sensitive and iron-resistant yeast strains. We observed that when the iron concentration in the medium increases, iron-sensitive strains accumulate iron more rapidly than iron-resistant isolates. We observed that, consistent with excess iron leading to oxidative stress, the redox state of iron-sensitive strains was more oxidized than that of iron-resistant strains. Growth assays in the presence of different oxidative reagents ruled out that this phenotype was due to alterations in the general oxidative stress protection machinery. It was noteworthy that iron-resistant strains were more sensitive to iron deficiency conditions than iron-sensitive strains, which suggests that adaptation to either high or low iron is detrimental for the opposite condition. An initial gene expression analysis suggested that alterations in iron homeostasis genes could contribute to the different responses of distant iron-sensitive and iron-resistant yeast strains to elevated environmental iron levels. This work has been funded by the AGL2011-29099 and BIO2014-56298-P grants from the Spanish Ministry of Economy and Competitiveness to S.P.; and the PROMETEOII/2014/042 grant from the Generalitat Valenciana to Dr. Amparo Querol. This work has also been supported by a predoctoral fellowship from the Spanish Ministry of Economy and Competitiveness to A.M.R., and a post-doctoral JAE-Doc contract from the Spanish Research Council (CSIC) and the European Social Fund to R.L. Peer reviewed 2016-04-28T11:17:37Z 2016-04-28T11:17:37Z 2016 artículo http://purl.org/coar/resource_type/c_6501 Applied and Environmental Microbiology 82 (6): 1906-1916 (2016) 0099-2240 http://hdl.handle.net/10261/131525 10.1128/AEM.03464-15 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 26773083 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-56298-P Postprint http://dx.doi.org/10.1128/AEM.03464-15 Sí open American Society for Microbiology
institution IATA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-iata-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IATA España
language English
topic Iron accumulation
Yeast
Oxidative stress
Homeostasis genes
Iron accumulation
Yeast
Oxidative stress
Homeostasis genes
spellingShingle Iron accumulation
Yeast
Oxidative stress
Homeostasis genes
Iron accumulation
Yeast
Oxidative stress
Homeostasis genes
Martínez Garay, Carlos Andrés
de Llanos, Rosa
Romero, Antonia
Martínez Pastor, M.Teresa
Puig, Sergi
Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
description Manuscrito correspondiente al artículo publicado con el título "Responses of Saccharomyces cerevisiae Strains from Different Origins to Elevated Iron Concentrations". -- Accepted manuscript posted online 15 January 2016
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Martínez Garay, Carlos Andrés
de Llanos, Rosa
Romero, Antonia
Martínez Pastor, M.Teresa
Puig, Sergi
format artículo
topic_facet Iron accumulation
Yeast
Oxidative stress
Homeostasis genes
author Martínez Garay, Carlos Andrés
de Llanos, Rosa
Romero, Antonia
Martínez Pastor, M.Teresa
Puig, Sergi
author_sort Martínez Garay, Carlos Andrés
title Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
title_short Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
title_full Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
title_fullStr Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
title_full_unstemmed Response to elevated iron concentrations in Saccharomyces cerevisiae strains of different origins
title_sort response to elevated iron concentrations in saccharomyces cerevisiae strains of different origins
publisher American Society for Microbiology
publishDate 2016
url http://hdl.handle.net/10261/131525
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
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AT dellanosrosa responsetoelevatedironconcentrationsinsaccharomycescerevisiaestrainsofdifferentorigins
AT romeroantonia responsetoelevatedironconcentrationsinsaccharomycescerevisiaestrainsofdifferentorigins
AT martinezpastormteresa responsetoelevatedironconcentrationsinsaccharomycescerevisiaestrainsofdifferentorigins
AT puigsergi responsetoelevatedironconcentrationsinsaccharomycescerevisiaestrainsofdifferentorigins
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