Mepanipyrim haptens and antibodies with nanomolar affinity
Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC50 = 3 nM). © 2013 The Royal Society of Chemistry.
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| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Published: |
Royal Society of Chemistry (UK)
2013
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| Subjects: | Mepanipyrim, Haptens, |
| Online Access: | http://hdl.handle.net/10261/110886 |
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