New transcriptomic insights in two RNAi wheat lines with the gliadins strongly down-regulated by two endosperm specific promoters
The gluten proteins of wheat grain are responsible for visco-elastic properties of flour, but they also trigger the immune-response of celiac disease. In this work, two low-gliadin RNA interference (RNAi) wheat lines that differ for the promoter driving the silencing (D-hordein and γ-gliadin promoters for D783 and D793 lines, respectively), were characterized at transcriptomic, and protein fraction levels in the grain. Silencing of gliadins provides a readjustment in the grain protein fractions that also affects to the non-gluten proteins (NGP), which were increased in both RNAi lines. Determination of wheat gluten by means of the R5 monoclonal antibody also showed a strong reduction in the content of gluten in both RNAi lines. Moreover, fructans, an oligosaccharide linked with the development of non-celiac wheat sensitivity (NCWS) were also significantly decreased in RNAi lines. The down-regulation of gliadins fractions also impacts to other metabolic processes, particularly on carbohydrate metabolism, enzyme regulator activity and response to stress. Genes and transcription factors regulated by ABA were up-regulated, which could suggest the implication of this phytohormone on the stress response observed in the RNAi lines.
Main Authors: | Marín-Sanz, Miriam, Masaru Iehisa, Julio C., Barro Losada, Francisco |
---|---|
Other Authors: | Ministerio de Ciencia e Innovación (España) |
Format: | artículo biblioteca |
Published: |
Elsevier
2022-02
|
Subjects: | Celiac disease, Gliadin, Protein compensation, RNAi lines, Triticum aestivum, |
Online Access: | http://hdl.handle.net/10261/271757 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
The α-Gliadins in Bread Wheat: Effect of Nitrogen Treatment on the Expression of the Major Celiac Disease Immunogenic Complex in Two RNAi Low-Gliadin Lines
by: Sánchez-León, Susana, et al.
Published: (2021-04-29) -
Oral Consumption of Bread from an RNAi Wheat Line with Strongly Silenced Gliadins Elicits No Immunogenic Response in a Pilot Study with Celiac Disease Patients
by: Guzmán-López, María H., et al.
Published: (2021-12-18) -
Prolamin Content and Grain Weight in RNAi Silenced Wheat Lines Under Different Conditions of Temperature and Nitrogen Availability
by: Marín-Sanz, Miriam, et al.
Published: (2020-03-20) -
Comparative proteomic analysis of two transgenic low-gliadin wheat lines and non-transgenic wheat control
by: García-Molina, María D., et al.
Published: (2017-08-08) -
Comparative study of the alpha-gliadin genes in diploid and polyploid genomes of wheat
by: Ozuna, Carmen, et al.
Published: (2013-10)