Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit
Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2015-02
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Subjects: | Antioxidant, l-ascorbate, l-galactose, d-galacturonic acid, Vitamin C, Gene expression, |
Online Access: | http://hdl.handle.net/10261/158869 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100000780 |
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dig-ias-es-10261-1588692020-05-28T07:56:16Z Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit Lado, Joanna Alós, Enriqueta Rodrigo, María Jesús Zacarías, Lorenzo European Commission Generalitat Valenciana Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Ministerio de Educación y Ciencia (España) Consejo Superior de Investigaciones Científicas (España) Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue. This work was supported by research grants FP7-PEOPLE-2011-CIG-303652 (Marie Curie Actions, European Union), Proyectos de I + D para Grupos de Investigación Emergentes GV/2012/036 (Generalitat Valenciana, Spain) and AGL 2012-34573 (CICyT, Ministerio de Educación y Ciencia, Spain). Financial support by PROMETEOII/2014/027 (Generalitat Valenciana) is also acknowledged. We acknowledge the Citrus Germplasm Bank for experimental material and the technical assistance of Amparo Beneyto. JL was recipient of a JAE-Predoc (CSIC-FSE) pre-doctoral contract and EA was recipient of a JAE-doc (CSIC-FSE) contract. Peer reviewed 2018-01-05T13:57:05Z 2018-01-05T13:57:05Z 2015-02 artículo http://purl.org/coar/resource_type/c_6501 Plant Science 231: 138-147 (2015) 0168-9452 http://hdl.handle.net/10261/158869 10.1016/j.plantsci.2014.12.002 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/303652 http://doi.org/10.1016/j.plantsci.2014.12.002 Sí none Elsevier |
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Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression |
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Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression Lado, Joanna Alós, Enriqueta Rodrigo, María Jesús Zacarías, Lorenzo Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
description |
Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue. |
author2 |
European Commission |
author_facet |
European Commission Lado, Joanna Alós, Enriqueta Rodrigo, María Jesús Zacarías, Lorenzo |
format |
artículo |
topic_facet |
Antioxidant l-ascorbate l-galactose d-galacturonic acid Vitamin C Gene expression |
author |
Lado, Joanna Alós, Enriqueta Rodrigo, María Jesús Zacarías, Lorenzo |
author_sort |
Lado, Joanna |
title |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
title_short |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
title_full |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
title_fullStr |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
title_full_unstemmed |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit |
title_sort |
light avoidance reduces ascorbic acid accumulation in the peel of citrus fruit |
publisher |
Elsevier |
publishDate |
2015-02 |
url |
http://hdl.handle.net/10261/158869 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100000780 |
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